A new inositol 1,4,5-trisphosphate binding protein similar to phospholipase C-δ1

Takashi Kanematsu, Yoshio Misumi, Yutaka Watanabe, Shoichiro Ozaki, Toshitaka Koga, Sadaaki Iwanaga, Yukio Ikehara, Masato Hirata

Research output: Contribution to journalArticle

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Abstract

We have reported that two inositol 1,4,5-trisphosphate binding proteins, with molecular masses of 85 and 130 kDa, were purified from rat brain; the former protein was found to be the δ1-isoenzyme of phospholipase C (PLC-δ1) and the latter was an unidentified novel protein [Kanematsu, Takeya, Watanabe, Ozaki, Yoshida, Koga, Iwanaga and Hirata (1992) J. Biol. Chem. 267, 6518-6525]. Here we describe the isolation of the full-length cDNA for the 130 kDa Ins(1,4,5)P3 binding protein, which encodes 1096 amino acids. The predicted sequence of the 130 kDa protein had 38.2% homology to that of PLC-δ1. Three known domains of PLC-δ1 (pleckstrin homology and putative catalytic X and Y domains) were located at residues 110-222, 377-544 and 585-804 with 35.2%, 48.2% and 45.8% homologies respectively. However, the protein showed no PLC activity to phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol. The 130 kDa protein expressed by transfection in COS-1 cells bound Ins(1,4,5)P3 in the same way as the molecule purified from brain. Thus the 130 kDa protein is a novel Ins(1,4,5)P3 binding protein homologous to PLC-δ1, but with no catalytic activity. The functional significance of the 130 kDa protein is discussed.

Original languageEnglish
Pages (from-to)319-325
Number of pages7
JournalBiochemical Journal
Volume313
Issue number1
DOIs
Publication statusPublished - Jan 1 1996

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Inositol 1,4,5-Trisphosphate
Type C Phospholipases
Carrier Proteins
Programmable logic controllers
Proteins
Phosphatidylinositols
Brain
COS Cells
Molecular mass
Isoenzymes
Transfection
Rats
Catalyst activity
Complementary DNA
Amino Acids
Molecules

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Kanematsu, T., Misumi, Y., Watanabe, Y., Ozaki, S., Koga, T., Iwanaga, S., ... Hirata, M. (1996). A new inositol 1,4,5-trisphosphate binding protein similar to phospholipase C-δ1 Biochemical Journal, 313(1), 319-325. https://doi.org/10.1042/bj3130319

A new inositol 1,4,5-trisphosphate binding protein similar to phospholipase C-δ1 . / Kanematsu, Takashi; Misumi, Yoshio; Watanabe, Yutaka; Ozaki, Shoichiro; Koga, Toshitaka; Iwanaga, Sadaaki; Ikehara, Yukio; Hirata, Masato.

In: Biochemical Journal, Vol. 313, No. 1, 01.01.1996, p. 319-325.

Research output: Contribution to journalArticle

Kanematsu, T, Misumi, Y, Watanabe, Y, Ozaki, S, Koga, T, Iwanaga, S, Ikehara, Y & Hirata, M 1996, 'A new inositol 1,4,5-trisphosphate binding protein similar to phospholipase C-δ1 ', Biochemical Journal, vol. 313, no. 1, pp. 319-325. https://doi.org/10.1042/bj3130319
Kanematsu, Takashi ; Misumi, Yoshio ; Watanabe, Yutaka ; Ozaki, Shoichiro ; Koga, Toshitaka ; Iwanaga, Sadaaki ; Ikehara, Yukio ; Hirata, Masato. / A new inositol 1,4,5-trisphosphate binding protein similar to phospholipase C-δ1 In: Biochemical Journal. 1996 ; Vol. 313, No. 1. pp. 319-325.
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