A new method for comprehensive bird's-eye analysis of the surgically excised internal limiting membrane

Toshio Hisatomi, Hiroshi Enaida, Taiji Sakamoto, Tadahisa Kagimoto, Akifumi Ueno, Takao Nakamura, Yasuaki Hata, Tatsuro Ishibashi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

PURPOSE: To investigate the role of cells and extracellular matrix on the internal limiting membrane (ILM), we demonstrated a new method for a comprehensive bird's-eye analysis of surgically excised ILM. DESIGN: Laboratory investigation. METHODS: The ILM of an idiopathic epiretinal membrane was fixed and spread out onto a glass slide, using fine needles under a biomicroscope. The expanded ILM was analyzed by light microscopy, immunohistochemistry, and scanning electron microscopy. RESULTS: The excised ILM could be unfolded and spread out as a flat sheet onto the glass slide. Immunohistochemistry revealed that most migrating cells were glial cells. Scanning electronmicroscopy revealed that the vitreous surface of the ILM was smooth, in contrast to the rough retinal surface. The ILM showed abundant cellular migration as a continuous stratified cellular sheet. CONCLUSIONS: This new bird's-eye-view observation of excised ILM enables us to carry out comprehensive analysis of cellular distribution from a temporal and spatial perspective.

Original languageEnglish
Pages (from-to)1121-1122
Number of pages2
JournalAmerican journal of ophthalmology
Volume139
Issue number6
DOIs
Publication statusPublished - Jun 1 2005

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Birds
Membranes
Glass
Immunohistochemistry
Epiretinal Membrane
Neuroglia
Electron Scanning Microscopy
Needles
Extracellular Matrix
Microscopy
Observation
Light

All Science Journal Classification (ASJC) codes

  • Ophthalmology

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A new method for comprehensive bird's-eye analysis of the surgically excised internal limiting membrane. / Hisatomi, Toshio; Enaida, Hiroshi; Sakamoto, Taiji; Kagimoto, Tadahisa; Ueno, Akifumi; Nakamura, Takao; Hata, Yasuaki; Ishibashi, Tatsuro.

In: American journal of ophthalmology, Vol. 139, No. 6, 01.06.2005, p. 1121-1122.

Research output: Contribution to journalArticle

Hisatomi, Toshio ; Enaida, Hiroshi ; Sakamoto, Taiji ; Kagimoto, Tadahisa ; Ueno, Akifumi ; Nakamura, Takao ; Hata, Yasuaki ; Ishibashi, Tatsuro. / A new method for comprehensive bird's-eye analysis of the surgically excised internal limiting membrane. In: American journal of ophthalmology. 2005 ; Vol. 139, No. 6. pp. 1121-1122.
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AU - Hisatomi, Toshio

AU - Enaida, Hiroshi

AU - Sakamoto, Taiji

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AU - Ueno, Akifumi

AU - Nakamura, Takao

AU - Hata, Yasuaki

AU - Ishibashi, Tatsuro

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AB - PURPOSE: To investigate the role of cells and extracellular matrix on the internal limiting membrane (ILM), we demonstrated a new method for a comprehensive bird's-eye analysis of surgically excised ILM. DESIGN: Laboratory investigation. METHODS: The ILM of an idiopathic epiretinal membrane was fixed and spread out onto a glass slide, using fine needles under a biomicroscope. The expanded ILM was analyzed by light microscopy, immunohistochemistry, and scanning electron microscopy. RESULTS: The excised ILM could be unfolded and spread out as a flat sheet onto the glass slide. Immunohistochemistry revealed that most migrating cells were glial cells. Scanning electronmicroscopy revealed that the vitreous surface of the ILM was smooth, in contrast to the rough retinal surface. The ILM showed abundant cellular migration as a continuous stratified cellular sheet. CONCLUSIONS: This new bird's-eye-view observation of excised ILM enables us to carry out comprehensive analysis of cellular distribution from a temporal and spatial perspective.

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