A New Method for Inoculation of Poor Germinator, Nosema sp. NIS M11 (Microsporida

Nosematidae), into an Insect Cell Culture

Chisa Yasunaga-Aoki, SHINO INOUE, MASAKO FUNAKOSHI, TAKESHI KAWARABATA, SYOJI HAYASAKA

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

ABSTRACT. Spores of Nosema sp. NIS M11, primed with 0.1 N KOH solution, were mixed with either physiological salt solutions or IPL‐41 medium, an insect cell culture medium, for germination. In the latter medium, only a few spores germinated, while high percentages of spore germination were obtained in physiological salt solutions, particularly in Rinaldini's solution. By using the salt solutions as inoculation media, KOH‐primed NIS M11 spores were inoculated into the Spodoptera frugiperda SF21AEII cell line. The initial infection levels were consistently higher than that obtained by using IPL‐41 medium. Among the salt solutions, Rinaldini's solution, containing KCl in place of NaCl, gave the highest percentage of initial cell infection. Increased osmolarity of salt solutions did not improve the efficiency of spore germination and infection of N. sp. NIS M11.

Original languageEnglish
Pages (from-to)191-195
Number of pages5
JournalJournal of Eukaryotic Microbiology
Volume42
Issue number2
DOIs
Publication statusPublished - Jan 1 1995

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Microsporida
Nosema
Insects
Cell Culture Techniques
Spores
Salts
Germination
Infection
Spodoptera
Osmolar Concentration
Culture Media

All Science Journal Classification (ASJC) codes

  • Microbiology

Cite this

A New Method for Inoculation of Poor Germinator, Nosema sp. NIS M11 (Microsporida : Nosematidae), into an Insect Cell Culture. / Yasunaga-Aoki, Chisa; INOUE, SHINO; FUNAKOSHI, MASAKO; KAWARABATA, TAKESHI; HAYASAKA, SYOJI.

In: Journal of Eukaryotic Microbiology, Vol. 42, No. 2, 01.01.1995, p. 191-195.

Research output: Contribution to journalArticle

Yasunaga-Aoki, Chisa ; INOUE, SHINO ; FUNAKOSHI, MASAKO ; KAWARABATA, TAKESHI ; HAYASAKA, SYOJI. / A New Method for Inoculation of Poor Germinator, Nosema sp. NIS M11 (Microsporida : Nosematidae), into an Insect Cell Culture. In: Journal of Eukaryotic Microbiology. 1995 ; Vol. 42, No. 2. pp. 191-195.
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