A new method for rapid cytotoxic T-lymphocyte induction using a multiple cytokine cocktail

Hideya Ohnishi, Norihiro Koya, Akifumi Kiyota, Hiroto Tanaka, Masayo Umebayashi, Mitsuo Katano, Takashi Morisaki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Immunotherapy using cytotoxic T-lymphocytes (CTLs) still has limited success. An increase in the frequency of CTL administration is one method to improve immunotherapy using CTLs. The conventional method (C-method) that generates CTLs after the induction of dendritic cells requires a long time period. If CTLs can be more rapidly and simply induced, the frequency of immunotherapy could be increased and unexpected contamination could be avoided. In this study, in order to more rapidly induce functional CTLs, we investigated a new method (N-method) that uses a cytokine cocktail, including interleukin (IL)-2, IL- 4, granulocyte macrophage colony-stimulating factor, tumour necrosis factor-α and interferon-α, together with a tumour lysate. CTLs induced by the N-method had equivalent functions, such as proliferation, surface antigen expression and cytotoxicity, compared with those induced by the C-method. These results suggest that the N-method can substitute the C-method in order to improve the effect of immunotherapy using CTLs.

Original languageEnglish
Pages (from-to)2385-2390
Number of pages6
JournalAnticancer Research
Volume32
Issue number6
Publication statusPublished - Jun 1 2012

Fingerprint

Cytotoxic T-Lymphocytes
Cytokines
Immunotherapy
Surface Antigens
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-4
Dendritic Cells
Interferons
Interleukin-2
Tumor Necrosis Factor-alpha

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Ohnishi, H., Koya, N., Kiyota, A., Tanaka, H., Umebayashi, M., Katano, M., & Morisaki, T. (2012). A new method for rapid cytotoxic T-lymphocyte induction using a multiple cytokine cocktail. Anticancer Research, 32(6), 2385-2390.

A new method for rapid cytotoxic T-lymphocyte induction using a multiple cytokine cocktail. / Ohnishi, Hideya; Koya, Norihiro; Kiyota, Akifumi; Tanaka, Hiroto; Umebayashi, Masayo; Katano, Mitsuo; Morisaki, Takashi.

In: Anticancer Research, Vol. 32, No. 6, 01.06.2012, p. 2385-2390.

Research output: Contribution to journalArticle

Ohnishi, H, Koya, N, Kiyota, A, Tanaka, H, Umebayashi, M, Katano, M & Morisaki, T 2012, 'A new method for rapid cytotoxic T-lymphocyte induction using a multiple cytokine cocktail', Anticancer Research, vol. 32, no. 6, pp. 2385-2390.
Ohnishi H, Koya N, Kiyota A, Tanaka H, Umebayashi M, Katano M et al. A new method for rapid cytotoxic T-lymphocyte induction using a multiple cytokine cocktail. Anticancer Research. 2012 Jun 1;32(6):2385-2390.
Ohnishi, Hideya ; Koya, Norihiro ; Kiyota, Akifumi ; Tanaka, Hiroto ; Umebayashi, Masayo ; Katano, Mitsuo ; Morisaki, Takashi. / A new method for rapid cytotoxic T-lymphocyte induction using a multiple cytokine cocktail. In: Anticancer Research. 2012 ; Vol. 32, No. 6. pp. 2385-2390.
@article{792dd2ad61fa4c9e9b7545c8c6c281d2,
title = "A new method for rapid cytotoxic T-lymphocyte induction using a multiple cytokine cocktail",
abstract = "Immunotherapy using cytotoxic T-lymphocytes (CTLs) still has limited success. An increase in the frequency of CTL administration is one method to improve immunotherapy using CTLs. The conventional method (C-method) that generates CTLs after the induction of dendritic cells requires a long time period. If CTLs can be more rapidly and simply induced, the frequency of immunotherapy could be increased and unexpected contamination could be avoided. In this study, in order to more rapidly induce functional CTLs, we investigated a new method (N-method) that uses a cytokine cocktail, including interleukin (IL)-2, IL- 4, granulocyte macrophage colony-stimulating factor, tumour necrosis factor-α and interferon-α, together with a tumour lysate. CTLs induced by the N-method had equivalent functions, such as proliferation, surface antigen expression and cytotoxicity, compared with those induced by the C-method. These results suggest that the N-method can substitute the C-method in order to improve the effect of immunotherapy using CTLs.",
author = "Hideya Ohnishi and Norihiro Koya and Akifumi Kiyota and Hiroto Tanaka and Masayo Umebayashi and Mitsuo Katano and Takashi Morisaki",
year = "2012",
month = "6",
day = "1",
language = "English",
volume = "32",
pages = "2385--2390",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "6",

}

TY - JOUR

T1 - A new method for rapid cytotoxic T-lymphocyte induction using a multiple cytokine cocktail

AU - Ohnishi, Hideya

AU - Koya, Norihiro

AU - Kiyota, Akifumi

AU - Tanaka, Hiroto

AU - Umebayashi, Masayo

AU - Katano, Mitsuo

AU - Morisaki, Takashi

PY - 2012/6/1

Y1 - 2012/6/1

N2 - Immunotherapy using cytotoxic T-lymphocytes (CTLs) still has limited success. An increase in the frequency of CTL administration is one method to improve immunotherapy using CTLs. The conventional method (C-method) that generates CTLs after the induction of dendritic cells requires a long time period. If CTLs can be more rapidly and simply induced, the frequency of immunotherapy could be increased and unexpected contamination could be avoided. In this study, in order to more rapidly induce functional CTLs, we investigated a new method (N-method) that uses a cytokine cocktail, including interleukin (IL)-2, IL- 4, granulocyte macrophage colony-stimulating factor, tumour necrosis factor-α and interferon-α, together with a tumour lysate. CTLs induced by the N-method had equivalent functions, such as proliferation, surface antigen expression and cytotoxicity, compared with those induced by the C-method. These results suggest that the N-method can substitute the C-method in order to improve the effect of immunotherapy using CTLs.

AB - Immunotherapy using cytotoxic T-lymphocytes (CTLs) still has limited success. An increase in the frequency of CTL administration is one method to improve immunotherapy using CTLs. The conventional method (C-method) that generates CTLs after the induction of dendritic cells requires a long time period. If CTLs can be more rapidly and simply induced, the frequency of immunotherapy could be increased and unexpected contamination could be avoided. In this study, in order to more rapidly induce functional CTLs, we investigated a new method (N-method) that uses a cytokine cocktail, including interleukin (IL)-2, IL- 4, granulocyte macrophage colony-stimulating factor, tumour necrosis factor-α and interferon-α, together with a tumour lysate. CTLs induced by the N-method had equivalent functions, such as proliferation, surface antigen expression and cytotoxicity, compared with those induced by the C-method. These results suggest that the N-method can substitute the C-method in order to improve the effect of immunotherapy using CTLs.

UR - http://www.scopus.com/inward/record.url?scp=84864534838&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84864534838&partnerID=8YFLogxK

M3 - Article

C2 - 22641679

AN - SCOPUS:84864534838

VL - 32

SP - 2385

EP - 2390

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 6

ER -