A new mitochondria-targeted ratiometric fluorescent probe based on diketopyrrolopyrrole for imaging endogenous HOCl in living cells

Xiaoxu Xie, Jian Wang, Yongchao Yan, Xiao Zhang, Chenchen Liu, Ji Yang, Jianli Hua

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Hypochlorous acid (HOCl) is a highly effective oxidant produced during phagocytosis and plays an important role in a variety of physiological and pathological processes. In this work, a new ratiometric fluorescent probe (PTZ-TDPP) based on a diketopyrrolopyrrole (DPP) and phenothiazine platform, was firstly designed and synthesized for the selective detection of HOCl. PTZ-TDPP showed an obvious color change from purple to pink along with a distinct fluorescence change from bright red to yellow in response to HOCl. The limit of detection towards HOCl was calculated to be 190 nM. For biological applications, PTZ-TDPP is of great development potential in detecting endogenous HOCl in living RAW 264.7 cells. A co-localization study employing MitoTracker Green showed that PTZ-TDPP was specifically located in the mitochondria of RAW 264.7 cells. Therefore, PTZ-TDPP has great potential for the detection of endogenous HOCl in living cells.

Original languageEnglish
Pages (from-to)5736-5743
Number of pages8
JournalAnalyst
Volume143
Issue number23
DOIs
Publication statusPublished - Dec 7 2018
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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