A new ‘paralyzed’ flagella mutant, OC‐10, in Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) that can be reactivated with adenosine triphosphate

Shogo Nakamura, Emiko Kawanishi, Soichi Nakamura, Shin Watanabe, Manabu K. Kojima

Research output: Contribution to journalArticle

Abstract

A new ‘paralyzed’ mutant. OC–10, was isolated in Chlamydomonas reinhardtii Dangeard. OC‐10 cannot swim and generally shows very little flagellar movement. However, when OC‐10 was demembranated, axonemal motility was reactivated in the presence of adenosine triphosphate (ATP) or adenosine diphosphate (ADP). The beating form of the reactivated axonemes was almost the same as that of the wild‐type axonemes. Flagellar regeneration of OC‐10 was slower than that of the wild‐type. Electron microscopic examination showed no abnormality in OC‐10 flagella, but SDS/PAGE revealed that mobility of a flagellar membrane protein was changed and a few bands disappeared in OC‐10 flagella, When the mutant was crossed to wild‐type to form temporary dikaryon cells with 4 flagella, OC‐10 flagella did not regain motility. Tetrad analysis of crosses between OC–10 and wild‐type demonstrated a 1:1 segregation on the basis of flagellar motility. From these results, we suppose that OC‐10 may be limited in ATP availability inside the flagella, or altered in flagellar membrane proteins important for motility.

Original languageEnglish
Pages (from-to)65-69
Number of pages5
JournalPhycological Research
Volume43
Issue number1
DOIs
Publication statusPublished - Mar 1995
Externally publishedYes

Fingerprint

Chlamydomonas reinhardtii
adenosine triphosphate
Chlorophyceae
Flagella
motility
flagellum
Adenosine Triphosphate
mutants
Axoneme
membrane proteins
membrane
Membrane Proteins
protein
adenosine diphosphate
abnormality
regeneration
Adenosine Diphosphate
Regeneration
Polyacrylamide Gel Electrophoresis
electron

All Science Journal Classification (ASJC) codes

  • Aquatic Science
  • Agricultural and Biological Sciences (miscellaneous)
  • Plant Science

Cite this

A new ‘paralyzed’ flagella mutant, OC‐10, in Chlamydomonas reinhardtii (Volvocales, Chlorophyceae) that can be reactivated with adenosine triphosphate. / Nakamura, Shogo; Kawanishi, Emiko; Nakamura, Soichi; Watanabe, Shin; Kojima, Manabu K.

In: Phycological Research, Vol. 43, No. 1, 03.1995, p. 65-69.

Research output: Contribution to journalArticle

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