A new polynucleotide-polysaccharide complex and its application to functional oligonucleotide delivery

Takahisa Anada, Kazuo Sakurai, Seiji Shinkai

Research output: Contribution to journalShort survey

1 Citation (Scopus)

Abstract

Schizophyllan (SPG) is a polysaccharide that belongs to the β-(1-3) glucan family and adopts a triple-helixical conformation in water. When SPG dissolves in dimethyl sulfoxide (DMSO), the triple helix is dissociated to three random coils. When water is added to the DMSO solution (renaturation), the single chain of SPG (s-SPG) collapses owing to both hydrophobic interaction and hydrogen bonding formation, and eventually aggregation takes place with increasing the water content. When this renaturation process is carried out in a mixture containing s-SPG and a single-stranded polynucleotide, a macro-molecular complex is formed, consisting of two s-SPG chains and one polynucleotide chain. This novel complexation was examined with circular dichroism, UV spectroscopy, and gel electrophoresis. We applied to this complex to deliver functional oligonucleotides such as antisense DNA and CpG motifs. The biological functions of these oligonucleotides were extremely enhanced owing to the complexation.

Original languageEnglish
Pages (from-to)49-57
Number of pages9
JournalTrends in Glycoscience and Glycotechnology
Volume17
Issue number94
DOIs
Publication statusPublished - Mar 2005

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Polynucleotides
Dimethyl Sulfoxide
Complexation
Oligonucleotides
Polysaccharides
Sizofiran
Antisense DNA
Glucans
Water
Electrophoresis
Ultraviolet spectroscopy
Water content
Macros
Conformations
Hydrogen bonds
Nucleotide Motifs
Agglomeration
Gels
Hydrogen Bonding
Circular Dichroism

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry

Cite this

A new polynucleotide-polysaccharide complex and its application to functional oligonucleotide delivery. / Anada, Takahisa; Sakurai, Kazuo; Shinkai, Seiji.

In: Trends in Glycoscience and Glycotechnology, Vol. 17, No. 94, 03.2005, p. 49-57.

Research output: Contribution to journalShort survey

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