A new reaction useful for chemical cross-linking between nucleic acids and proteins

Noriko Nitta, Osamu Kuge, Seiko Yui, Akiko Tsugawa, Kazuo Negishi, Hikoya Hayatsu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Cytosine in nucleic acids can be converted into N4-aminocytosine by treatment with a mixture of hydrazine and bisulfite. The hydrazino group thus formed at position 4 of the pyrimidine ring can be linked to a sulhydryl group in proteins by the use of bromopyruvate as a linker. Successful use of this scheme of chemical cross-linking between nucleic acid and protein was demonstrated in the linking of poly(C) with glutathione, and of RNA with protein in the E. coli 30 S ribosomal subunit.

Original languageEnglish
Pages (from-to)194-198
Number of pages5
JournalFEBS Letters
Volume166
Issue number1
DOIs
Publication statusPublished - Jan 23 1984
Externally publishedYes

Fingerprint

hydrazine
Nucleic Acids
Poly C
Ribosome Subunits
Escherichia coli Proteins
Cytosine
Glutathione
Proteins
RNA
Escherichia coli
bromopyruvate
pyrimidine
hydrogen sulfite

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

A new reaction useful for chemical cross-linking between nucleic acids and proteins. / Nitta, Noriko; Kuge, Osamu; Yui, Seiko; Tsugawa, Akiko; Negishi, Kazuo; Hayatsu, Hikoya.

In: FEBS Letters, Vol. 166, No. 1, 23.01.1984, p. 194-198.

Research output: Contribution to journalArticle

Nitta, Noriko ; Kuge, Osamu ; Yui, Seiko ; Tsugawa, Akiko ; Negishi, Kazuo ; Hayatsu, Hikoya. / A new reaction useful for chemical cross-linking between nucleic acids and proteins. In: FEBS Letters. 1984 ; Vol. 166, No. 1. pp. 194-198.
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