Abstract
Cytosine in nucleic acids can be converted into N4-aminocytosine by treatment with a mixture of hydrazine and bisulfite. The hydrazino group thus formed at position 4 of the pyrimidine ring can be linked to a sulhydryl group in proteins by the use of bromopyruvate as a linker. Successful use of this scheme of chemical cross-linking between nucleic acid and protein was demonstrated in the linking of poly(C) with glutathione, and of RNA with protein in the E. coli 30 S ribosomal subunit.
| Original language | English |
|---|---|
| Pages (from-to) | 194-198 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 166 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 23 1984 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology
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A new reaction useful for chemical cross-linking between nucleic acids and proteins. / Nitta, Noriko; Kuge, Osamu; Yui, Seiko; Tsugawa, Akiko; Negishi, Kazuo; Hayatsu, Hikoya.
In: FEBS Letters, Vol. 166, No. 1, 23.01.1984, p. 194-198.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A new reaction useful for chemical cross-linking between nucleic acids and proteins
AU - Nitta, Noriko
AU - Kuge, Osamu
AU - Yui, Seiko
AU - Tsugawa, Akiko
AU - Negishi, Kazuo
AU - Hayatsu, Hikoya
PY - 1984/1/23
Y1 - 1984/1/23
N2 - Cytosine in nucleic acids can be converted into N4-aminocytosine by treatment with a mixture of hydrazine and bisulfite. The hydrazino group thus formed at position 4 of the pyrimidine ring can be linked to a sulhydryl group in proteins by the use of bromopyruvate as a linker. Successful use of this scheme of chemical cross-linking between nucleic acid and protein was demonstrated in the linking of poly(C) with glutathione, and of RNA with protein in the E. coli 30 S ribosomal subunit.
AB - Cytosine in nucleic acids can be converted into N4-aminocytosine by treatment with a mixture of hydrazine and bisulfite. The hydrazino group thus formed at position 4 of the pyrimidine ring can be linked to a sulhydryl group in proteins by the use of bromopyruvate as a linker. Successful use of this scheme of chemical cross-linking between nucleic acid and protein was demonstrated in the linking of poly(C) with glutathione, and of RNA with protein in the E. coli 30 S ribosomal subunit.
UR - http://www.scopus.com/inward/record.url?scp=0021769146&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021769146&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(84)80071-X
DO - 10.1016/0014-5793(84)80071-X
M3 - Article
C2 - 6692920
AN - SCOPUS:0021769146
VL - 166
SP - 194
EP - 198
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -