A new reaction useful for chemical cross-linking between nucleic acids and proteins

Noriko Nitta, Osamu Kuge, Seiko Yui, Akiko Tsugawa, Kazuo Negishi, Hikoya Hayatsu

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Cytosine in nucleic acids can be converted into N4-aminocytosine by treatment with a mixture of hydrazine and bisulfite. The hydrazino group thus formed at position 4 of the pyrimidine ring can be linked to a sulhydryl group in proteins by the use of bromopyruvate as a linker. Successful use of this scheme of chemical cross-linking between nucleic acid and protein was demonstrated in the linking of poly(C) with glutathione, and of RNA with protein in the E. coli 30 S ribosomal subunit.

Original languageEnglish
Pages (from-to)194-198
Number of pages5
JournalFEBS Letters
Volume166
Issue number1
DOIs
Publication statusPublished - Jan 23 1984
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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