A New Sulfonated Tetrazolium Salt That Produces a Highly Water-Soluble Formazan Dye

Munetaka Ishiyama, Masanobu Shiga, Kazumi Sasamoto, Makoto Mizoguchi, Pingang He

Research output: Contribution to journalArticle

440 Citations (Scopus)

Abstract

A new tetrazolium compound, sodium salt of 4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-l,3-benzene disulfonate (la), which produces a highly water-soluble formazan dye, due to the presence of two sulfonate groups, was synthesized and its potential utility evaluated in assays of NADH and cell proliferation. The compound proved to have a similar sensitivity to 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) in the assay of NADH and was also useful as an indicator of cell viability, with less cytotoxicity than XTT, in the proliferation assay using P388 cell lines.

Original languageEnglish
Pages (from-to)1118-1122
Number of pages5
JournalChemical and Pharmaceutical Bulletin
Volume41
Issue number6
DOIs
Publication statusPublished - Jan 1 1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Drug Discovery

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