TY - JOUR
T1 - A new trisaccharide sugar chain linked to a serine residue in bovine blood coagulation factors VII and IX
AU - Hase, Sumihiro
AU - Kawabata, Shun ichiro
AU - Nishimura, Hitoshi
AU - Takeya, Hiroyuki
AU - Sueyoshi, Tatsuya
AU - Miyata, Toshiyuki
AU - Iwanaga, Sadaaki
AU - Takao, Toshifumi
AU - Shimonishi, Yasutsugu
AU - Ikenaka, Tokuji
PY - 1988/12
Y1 - 1988/12
N2 - A new trisaccharide sugar chain was identified in bovine blood coagulation factors VII and IX. A pentapeptide isolated from factor VII contained Ser-52, which could not be identified with a gas-phase sequencer, suggesting an unknown substituent on the serine residue (Takeya, H. et al. (1988) J. Biol. Chem., in press). The same results were obtained for a pentapeptide containing Ser-53 of factor IX. Component sugar analysis revealed that the peptide contained 1 mol of glucose and 2 mol of xylose. This sugar component was also confirmed by high-resolution fast atom bombardment mass spectrometric analysis of the pentapeptide. The trisaccharide was released from the peptides by means of β-elimination reaction and its reducing end was coupled with 2-aminopyridine. The fluorescent pyridylamino (PA-) derivative of the trisaccharide was purified by gel-filtration and reversed-phase HPLC. The sugar composition of the PA-trisaccharide was found to be 2 mol of xylose and 1 mol of PA-glucose. These results indicate the existence of a (Xyl2)Glc-Ser structure in factors VII and IX.
AB - A new trisaccharide sugar chain was identified in bovine blood coagulation factors VII and IX. A pentapeptide isolated from factor VII contained Ser-52, which could not be identified with a gas-phase sequencer, suggesting an unknown substituent on the serine residue (Takeya, H. et al. (1988) J. Biol. Chem., in press). The same results were obtained for a pentapeptide containing Ser-53 of factor IX. Component sugar analysis revealed that the peptide contained 1 mol of glucose and 2 mol of xylose. This sugar component was also confirmed by high-resolution fast atom bombardment mass spectrometric analysis of the pentapeptide. The trisaccharide was released from the peptides by means of β-elimination reaction and its reducing end was coupled with 2-aminopyridine. The fluorescent pyridylamino (PA-) derivative of the trisaccharide was purified by gel-filtration and reversed-phase HPLC. The sugar composition of the PA-trisaccharide was found to be 2 mol of xylose and 1 mol of PA-glucose. These results indicate the existence of a (Xyl2)Glc-Ser structure in factors VII and IX.
UR - http://www.scopus.com/inward/record.url?scp=0024270443&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024270443&partnerID=8YFLogxK
U2 - 10.1093/oxfordjournals.jbchem.a122571
DO - 10.1093/oxfordjournals.jbchem.a122571
M3 - Article
C2 - 3149637
AN - SCOPUS:0024270443
SN - 0021-924X
VL - 104
SP - 867
EP - 868
JO - Journal of Biochemistry
JF - Journal of Biochemistry
IS - 6
ER -