A new trisaccharide sugar chain linked to a serine residue in bovine blood coagulation factors VII and IX

Sumihiro Hase, Shun ichiro Kawabata, Hitoshi Nishimura, Hiroyuki Takeya, Tatsuya Sueyoshi, Toshiyuki Miyata, Sadaaki Iwanaga, Toshifumi Takao, Yasutsugu Shimonishi, Tokuji Ikenaka

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Abstract

A new trisaccharide sugar chain was identified in bovine blood coagulation factors VII and IX. A pentapeptide isolated from factor VII contained Ser-52, which could not be identified with a gas-phase sequencer, suggesting an unknown substituent on the serine residue (Takeya, H. et al. (1988) J. Biol. Chem., in press). The same results were obtained for a pentapeptide containing Ser-53 of factor IX. Component sugar analysis revealed that the peptide contained 1 mol of glucose and 2 mol of xylose. This sugar component was also confirmed by high-resolution fast atom bombardment mass spectrometric analysis of the pentapeptide. The trisaccharide was released from the peptides by means of β-elimination reaction and its reducing end was coupled with 2-aminopyridine. The fluorescent pyridylamino (PA-) derivative of the trisaccharide was purified by gel-filtration and reversed-phase HPLC. The sugar composition of the PA-trisaccharide was found to be 2 mol of xylose and 1 mol of PA-glucose. These results indicate the existence of a (Xyl2)Glc-Ser structure in factors VII and IX.

Original languageEnglish
Pages (from-to)867-868
Number of pages2
JournalJournal of biochemistry
Volume104
Issue number6
DOIs
Publication statusPublished - Dec 1988

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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    Hase, S., Kawabata, S. I., Nishimura, H., Takeya, H., Sueyoshi, T., Miyata, T., Iwanaga, S., Takao, T., Shimonishi, Y., & Ikenaka, T. (1988). A new trisaccharide sugar chain linked to a serine residue in bovine blood coagulation factors VII and IX. Journal of biochemistry, 104(6), 867-868. https://doi.org/10.1093/oxfordjournals.jbchem.a122571