A Non-α-Like DNA Polymerase from the Hyperthermophilic Archaeon Pyrococcus Furiosus

Mitsuo Imamura, Takashi Uemori, Ikunoshin Kato, Yoshizumi Ishino

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We identified a DNA polymerase with properties that differed from those of α-like DNA polymerase (Pol I), in Pyrococcus furiosus, a hyperthermophilic archaeon. The novel DNA polymerase (Pol II) was partially purified and characterized. The DNA polymerizing activity of Pol II was relatively sensitive to dideoxythymidine-triphosphate (ddTTP) and it was inhibited by N-ethylmaleimide, but not by aphidicolin. Activity staining gel electrophoresis showed that the DNA polymerizing activity was derived from a polypeptide with a molecular weight of 130000 on sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. The DNA elongation ability of Pol II using a natural DNA template was much lower than that of Pol I from this organism and DNA synthesis of Pol II seems to be non-processive.

Original languageEnglish
Pages (from-to)1647-1652
Number of pages6
JournalBiological and Pharmaceutical Bulletin
Volume18
Issue number12
DOIs
Publication statusPublished - Jan 1 1995

Fingerprint

Pyrococcus furiosus
DNA Polymerase II
Archaea
DNA-Directed DNA Polymerase
DNA Polymerase I
DNA
Aphidicolin
Ethylmaleimide
Sodium Dodecyl Sulfate
Electrophoresis
Polyacrylamide Gel Electrophoresis
Molecular Weight
Gels
Staining and Labeling
Peptides

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

Cite this

A Non-α-Like DNA Polymerase from the Hyperthermophilic Archaeon Pyrococcus Furiosus. / Imamura, Mitsuo; Uemori, Takashi; Kato, Ikunoshin; Ishino, Yoshizumi.

In: Biological and Pharmaceutical Bulletin, Vol. 18, No. 12, 01.01.1995, p. 1647-1652.

Research output: Contribution to journalArticle

Imamura, Mitsuo ; Uemori, Takashi ; Kato, Ikunoshin ; Ishino, Yoshizumi. / A Non-α-Like DNA Polymerase from the Hyperthermophilic Archaeon Pyrococcus Furiosus. In: Biological and Pharmaceutical Bulletin. 1995 ; Vol. 18, No. 12. pp. 1647-1652.
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