A Non-α-Like DNA Polymerase from the Hyperthermophilic Archaeon Pyrococcus Furiosus

Mitsuo Imamura, Takashi Uemori, Ikunoshin Kato, Yoshizumi Ishino

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

We identified a DNA polymerase with properties that differed from those of α-like DNA polymerase (Pol I), in Pyrococcus furiosus, a hyperthermophilic archaeon. The novel DNA polymerase (Pol II) was partially purified and characterized. The DNA polymerizing activity of Pol II was relatively sensitive to dideoxythymidine-triphosphate (ddTTP) and it was inhibited by N-ethylmaleimide, but not by aphidicolin. Activity staining gel electrophoresis showed that the DNA polymerizing activity was derived from a polypeptide with a molecular weight of 130000 on sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. The DNA elongation ability of Pol II using a natural DNA template was much lower than that of Pol I from this organism and DNA synthesis of Pol II seems to be non-processive.

Original languageEnglish
Pages (from-to)1647-1652
Number of pages6
JournalBiological and Pharmaceutical Bulletin
Volume18
Issue number12
DOIs
Publication statusPublished - 1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

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