A novel 550-kDa protein in skeletal muscle of chick embryo: purification and localization

Akihito Hattori, Jun ichi Wakamatsu, Tatsuo Ishii, Kyoko Kuwahara, Ryuichi Tatsumi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have found a novel protein with a molecular mass of 550 kDa on SDS-polyacrylamide gels, which is abundant in skeletal muscle tissues at an early stage of chick embryonic development. The 550-kDa protein decreased with the progress of development, and only a slight amount of the protein was present in adult chicken skeletal muscle. The 550-kDa protein was purified from the cytoplasm of 18 day embryos by a procedure including ultracentrifugation and gel filtration. The purified 550-kDa protein was essentially free of contaminants as judged by SDS-PAGE. By immunofluorescence and immunoelectron microscopy using the antibody raised against the 550-kDa protein, this protein was shown to be localized in the peripheries of adult muscle fibers and at the Z-disks of isolated myofibrils. These findings have led us to conclude that the 550-kDa protein is a novel myofibrillar protein in chicken skeletal muscle.

Original languageEnglish
Pages (from-to)191-200
Number of pages10
JournalBBA - General Subjects
Volume1245
Issue number2
DOIs
Publication statusPublished - Oct 19 1995
Externally publishedYes

Fingerprint

Chick Embryo
Purification
Muscle
Skeletal Muscle
Proteins
Chickens
Muscles
Immunoelectron Microscopy
Myofibrils
Ultracentrifugation
Molecular mass
Fluorescence Microscopy
Embryonic Development
Gel Chromatography
Polyacrylamide Gel Electrophoresis
Microscopic examination
Cytoplasm
Embryonic Structures
Gels
Impurities

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

A novel 550-kDa protein in skeletal muscle of chick embryo : purification and localization. / Hattori, Akihito; Wakamatsu, Jun ichi; Ishii, Tatsuo; Kuwahara, Kyoko; Tatsumi, Ryuichi.

In: BBA - General Subjects, Vol. 1245, No. 2, 19.10.1995, p. 191-200.

Research output: Contribution to journalArticle

Hattori, Akihito ; Wakamatsu, Jun ichi ; Ishii, Tatsuo ; Kuwahara, Kyoko ; Tatsumi, Ryuichi. / A novel 550-kDa protein in skeletal muscle of chick embryo : purification and localization. In: BBA - General Subjects. 1995 ; Vol. 1245, No. 2. pp. 191-200.
@article{36c6ee4a00874031826a382b20b77def,
title = "A novel 550-kDa protein in skeletal muscle of chick embryo: purification and localization",
abstract = "We have found a novel protein with a molecular mass of 550 kDa on SDS-polyacrylamide gels, which is abundant in skeletal muscle tissues at an early stage of chick embryonic development. The 550-kDa protein decreased with the progress of development, and only a slight amount of the protein was present in adult chicken skeletal muscle. The 550-kDa protein was purified from the cytoplasm of 18 day embryos by a procedure including ultracentrifugation and gel filtration. The purified 550-kDa protein was essentially free of contaminants as judged by SDS-PAGE. By immunofluorescence and immunoelectron microscopy using the antibody raised against the 550-kDa protein, this protein was shown to be localized in the peripheries of adult muscle fibers and at the Z-disks of isolated myofibrils. These findings have led us to conclude that the 550-kDa protein is a novel myofibrillar protein in chicken skeletal muscle.",
author = "Akihito Hattori and Wakamatsu, {Jun ichi} and Tatsuo Ishii and Kyoko Kuwahara and Ryuichi Tatsumi",
year = "1995",
month = "10",
day = "19",
doi = "10.1016/0304-4165(95)00096-T",
language = "English",
volume = "1245",
pages = "191--200",
journal = "Biochimica et Biophysica Acta - General Subjects",
issn = "0304-4165",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - A novel 550-kDa protein in skeletal muscle of chick embryo

T2 - purification and localization

AU - Hattori, Akihito

AU - Wakamatsu, Jun ichi

AU - Ishii, Tatsuo

AU - Kuwahara, Kyoko

AU - Tatsumi, Ryuichi

PY - 1995/10/19

Y1 - 1995/10/19

N2 - We have found a novel protein with a molecular mass of 550 kDa on SDS-polyacrylamide gels, which is abundant in skeletal muscle tissues at an early stage of chick embryonic development. The 550-kDa protein decreased with the progress of development, and only a slight amount of the protein was present in adult chicken skeletal muscle. The 550-kDa protein was purified from the cytoplasm of 18 day embryos by a procedure including ultracentrifugation and gel filtration. The purified 550-kDa protein was essentially free of contaminants as judged by SDS-PAGE. By immunofluorescence and immunoelectron microscopy using the antibody raised against the 550-kDa protein, this protein was shown to be localized in the peripheries of adult muscle fibers and at the Z-disks of isolated myofibrils. These findings have led us to conclude that the 550-kDa protein is a novel myofibrillar protein in chicken skeletal muscle.

AB - We have found a novel protein with a molecular mass of 550 kDa on SDS-polyacrylamide gels, which is abundant in skeletal muscle tissues at an early stage of chick embryonic development. The 550-kDa protein decreased with the progress of development, and only a slight amount of the protein was present in adult chicken skeletal muscle. The 550-kDa protein was purified from the cytoplasm of 18 day embryos by a procedure including ultracentrifugation and gel filtration. The purified 550-kDa protein was essentially free of contaminants as judged by SDS-PAGE. By immunofluorescence and immunoelectron microscopy using the antibody raised against the 550-kDa protein, this protein was shown to be localized in the peripheries of adult muscle fibers and at the Z-disks of isolated myofibrils. These findings have led us to conclude that the 550-kDa protein is a novel myofibrillar protein in chicken skeletal muscle.

UR - http://www.scopus.com/inward/record.url?scp=0028790461&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028790461&partnerID=8YFLogxK

U2 - 10.1016/0304-4165(95)00096-T

DO - 10.1016/0304-4165(95)00096-T

M3 - Article

C2 - 7492577

AN - SCOPUS:0028790461

VL - 1245

SP - 191

EP - 200

JO - Biochimica et Biophysica Acta - General Subjects

JF - Biochimica et Biophysica Acta - General Subjects

SN - 0304-4165

IS - 2

ER -