A novel Alaska pollack-derived peptide, which increases glucose uptake in skeletal muscle cells, lowers the blood glucose level in diabetic mice

Tatsuhiro Ayabe, Takafumi Mizushige, Wakana Ota, Fuminori Kawabata, Kohsuke Hayamizu, Li Han, Tomoko Tsuji, Ryuhei Kanamoto, Kousaku Ohinata

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6 Citations (Scopus)


We found that the tryptic digest of Alaska pollack protein exhibits a glucose-lowering effect in KK-Ay mice, a type II diabetic model. We then searched for glucose-lowering peptides in the digest. Ala-Asn-Gly-Glu-Val-Ala-Gln-Trp-Arg (ANGEVAQWR) was identified from a peak of the HPLC fraction selected based on the glucose-lowering activity in an insulin resistance test using ddY mice. ANGEVAQWR (3 mg kg-1) decreased the blood glucose level after intraperitoneal administration. Among its fragment peptides, the C-terminal tripeptide, Gln-Trp-Arg (QWR, 1 mg kg-1), lowered the blood glucose level, suggesting that the C-terminal is critical for glucose-lowering activity. QWR also enhanced glucose uptake into C2C12, a mouse skeletal muscle cell line. QWR did not induce the phosphorylation of serine/threonine protein kinase B (Akt) and adenosine monophosphate-activated protein kinase (AMPK). We also demonstrated that QWR lowered the blood glucose level in NSY and KK-Ay, type II diabetic models.

Original languageEnglish
Pages (from-to)2749-2757
Number of pages9
JournalFood and Function
Issue number8
Publication statusPublished - Aug 1 2015


All Science Journal Classification (ASJC) codes

  • Food Science

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