A novel analytical ELISA-based methodology for pharmacologically active saikosaponins

S. Zhu, S. Shimokawa, Y. Shoyama, Hiroyuki Tanaka

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A competitive enzyme-linked immunosorbent assay (ELISA) for saikosaponins was established using monoclonal antibody (MAb) 3G10. Hybridoma 3G10 prepared by fusing splenocytes immunized with saikosaponin a-BSA (SSa-BSA) conjugate and a hypoxanthine-aminopterin-thymidine (HAT)-sensitive mouse myeloma cell line, P3-X63-Ag8-U1, secreted monoclonal antibodies with wide cross-reactivity to saikosaponins including saikosaponin b2 (SSb2), c (SSc) and d (SSd), which are stereo and/or regio isomers of SSa. The method, at an effective measuring range of 0.6 μg /ml to 2.3 μg/ml of SSa, successfully detected total saikosaponins in Bupleuri radix and Kampo medicines prescribed with Bupleuri radix. Good correlation between ELISA and HPLC analyses of total saikosaponin in a crude extract of Bupleuri radix was obtained after hydrolysis of acyl saikosaponins by treatment with a mild alkaline solution.

Original languageEnglish
Pages (from-to)100-108
Number of pages9
JournalFitoterapia
Volume77
Issue number2
DOIs
Publication statusPublished - Feb 1 2006

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Enzyme-Linked Immunosorbent Assay
Aminopterin
Monoclonal Antibodies
Kampo Medicine
Hypoxanthine
Hybridomas
saikosaponin
Complex Mixtures
Thymidine
Hydrolysis
High Pressure Liquid Chromatography
Cell Line

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

Cite this

A novel analytical ELISA-based methodology for pharmacologically active saikosaponins. / Zhu, S.; Shimokawa, S.; Shoyama, Y.; Tanaka, Hiroyuki.

In: Fitoterapia, Vol. 77, No. 2, 01.02.2006, p. 100-108.

Research output: Contribution to journalArticle

Zhu, S. ; Shimokawa, S. ; Shoyama, Y. ; Tanaka, Hiroyuki. / A novel analytical ELISA-based methodology for pharmacologically active saikosaponins. In: Fitoterapia. 2006 ; Vol. 77, No. 2. pp. 100-108.
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