A novel analytical ELISA-based methodology for pharmacologically active saikosaponins

S. Zhu, S. Shimokawa, Y. Shoyama, H. Tanaka

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

A competitive enzyme-linked immunosorbent assay (ELISA) for saikosaponins was established using monoclonal antibody (MAb) 3G10. Hybridoma 3G10 prepared by fusing splenocytes immunized with saikosaponin a-BSA (SSa-BSA) conjugate and a hypoxanthine-aminopterin-thymidine (HAT)-sensitive mouse myeloma cell line, P3-X63-Ag8-U1, secreted monoclonal antibodies with wide cross-reactivity to saikosaponins including saikosaponin b2 (SSb2), c (SSc) and d (SSd), which are stereo and/or regio isomers of SSa. The method, at an effective measuring range of 0.6 μg /ml to 2.3 μg/ml of SSa, successfully detected total saikosaponins in Bupleuri radix and Kampo medicines prescribed with Bupleuri radix. Good correlation between ELISA and HPLC analyses of total saikosaponin in a crude extract of Bupleuri radix was obtained after hydrolysis of acyl saikosaponins by treatment with a mild alkaline solution.

Original languageEnglish
Pages (from-to)100-108
Number of pages9
JournalFitoterapia
Volume77
Issue number2
DOIs
Publication statusPublished - Feb 1 2006

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

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