A novel approach of optical biopsy using probe-based confocal laser endomicroscopy for peritoneal metastasis

Hisashi Hara, Tsuyoshi Takahashi, Rie Nakatsuka, Shigeyoshi Higashi, Tetsuji Naka, Kazuki Sumiyama, Yasuhiro Miyazaki, Tomoki Makino, Yukinori Kurokawa, Makoto Yamasaki, Shuji Takiguchi, Masaki Mori, Yuichiro Doki, Kiyokazu Nakajima

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: In digestive cancers, it is mandatory to diagnose peritoneal metastasis prior to selecting therapy. Therefore, exploratory laparoscopy has gained wider clinical acceptance. In laparoscopy, the peritoneal metastasis is pathologically confirmed by excisional biopsy; however, there remain technical difficulties in performing precise diagnosis and adequate biopsy on small peritoneal lesions without damaging organs. We have focused on “optical biopsy” using probe-based confocal laser endomicroscopy (pCLE). The aims of this study were (1) to optimize current CLE system for real-time observation of peritoneal metastases and (2) to assess its potential usefulness as diagnostic modality in preclinical settings. Methods: To optimize condition and evaluate feasibility, we prepared peritoneal metastasis mice model with gastric cancer cell line (MKN-45). On Day 10 after seeding, the mice were laparotomized and performed pCLE observations with CellvizioLAB (LSU-F 400/488 nm, Mauna Kea Technologies, Paris, France). We evaluated two different CLE probes, three different dyes, and optimal interval time. The detected sites were excised and pathologically evaluated on its morphology. Next, the feasibility and safety were validated in porcine model for clinical usage. After injection of fluorescein, pCLE was applied for the observation of intra-abdominal organs. Result: A miniature probe-type pCLE system with 60 μm focal depth (UltraMini O) and 1 % fluorescein dye was chosen for good visualization in mice model. The irregular microarchitecture images suspected to malignancy were obtained from the metastases. In the porcine model, observation of abdominal organs was feasible without any organ injury in the laparoscopic procedures. The dosage of 1 % fluorescein (3 ml/body) was appropriate in observing intra-abdominal organs, and each intra-abdominal organ was clearly observed with the same imaging quality we obtained in mice model. Conclusion: The pCLE was feasible and safe and potentially useful for the diagnosis of the peritoneal metastasis in in vivo animal models.

Original languageEnglish
Pages (from-to)3437-3446
Number of pages10
JournalSurgical endoscopy
Volume30
Issue number8
DOIs
Publication statusPublished - Aug 1 2016
Externally publishedYes

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Lasers
Neoplasm Metastasis
Biopsy
Fluorescein
Observation
Laparoscopy
Coloring Agents
Swine
Computer Systems
Paris
Stomach Neoplasms
France
Neoplasms
Animal Models
Technology
Safety
Cell Line
Injections
Wounds and Injuries
Therapeutics

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Hara, H., Takahashi, T., Nakatsuka, R., Higashi, S., Naka, T., Sumiyama, K., ... Nakajima, K. (2016). A novel approach of optical biopsy using probe-based confocal laser endomicroscopy for peritoneal metastasis. Surgical endoscopy, 30(8), 3437-3446. https://doi.org/10.1007/s00464-015-4626-7

A novel approach of optical biopsy using probe-based confocal laser endomicroscopy for peritoneal metastasis. / Hara, Hisashi; Takahashi, Tsuyoshi; Nakatsuka, Rie; Higashi, Shigeyoshi; Naka, Tetsuji; Sumiyama, Kazuki; Miyazaki, Yasuhiro; Makino, Tomoki; Kurokawa, Yukinori; Yamasaki, Makoto; Takiguchi, Shuji; Mori, Masaki; Doki, Yuichiro; Nakajima, Kiyokazu.

In: Surgical endoscopy, Vol. 30, No. 8, 01.08.2016, p. 3437-3446.

Research output: Contribution to journalArticle

Hara, H, Takahashi, T, Nakatsuka, R, Higashi, S, Naka, T, Sumiyama, K, Miyazaki, Y, Makino, T, Kurokawa, Y, Yamasaki, M, Takiguchi, S, Mori, M, Doki, Y & Nakajima, K 2016, 'A novel approach of optical biopsy using probe-based confocal laser endomicroscopy for peritoneal metastasis', Surgical endoscopy, vol. 30, no. 8, pp. 3437-3446. https://doi.org/10.1007/s00464-015-4626-7
Hara, Hisashi ; Takahashi, Tsuyoshi ; Nakatsuka, Rie ; Higashi, Shigeyoshi ; Naka, Tetsuji ; Sumiyama, Kazuki ; Miyazaki, Yasuhiro ; Makino, Tomoki ; Kurokawa, Yukinori ; Yamasaki, Makoto ; Takiguchi, Shuji ; Mori, Masaki ; Doki, Yuichiro ; Nakajima, Kiyokazu. / A novel approach of optical biopsy using probe-based confocal laser endomicroscopy for peritoneal metastasis. In: Surgical endoscopy. 2016 ; Vol. 30, No. 8. pp. 3437-3446.
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abstract = "Background: In digestive cancers, it is mandatory to diagnose peritoneal metastasis prior to selecting therapy. Therefore, exploratory laparoscopy has gained wider clinical acceptance. In laparoscopy, the peritoneal metastasis is pathologically confirmed by excisional biopsy; however, there remain technical difficulties in performing precise diagnosis and adequate biopsy on small peritoneal lesions without damaging organs. We have focused on “optical biopsy” using probe-based confocal laser endomicroscopy (pCLE). The aims of this study were (1) to optimize current CLE system for real-time observation of peritoneal metastases and (2) to assess its potential usefulness as diagnostic modality in preclinical settings. Methods: To optimize condition and evaluate feasibility, we prepared peritoneal metastasis mice model with gastric cancer cell line (MKN-45). On Day 10 after seeding, the mice were laparotomized and performed pCLE observations with CellvizioLAB (LSU-F 400/488 nm, Mauna Kea Technologies, Paris, France). We evaluated two different CLE probes, three different dyes, and optimal interval time. The detected sites were excised and pathologically evaluated on its morphology. Next, the feasibility and safety were validated in porcine model for clinical usage. After injection of fluorescein, pCLE was applied for the observation of intra-abdominal organs. Result: A miniature probe-type pCLE system with 60 μm focal depth (UltraMini O) and 1 {\%} fluorescein dye was chosen for good visualization in mice model. The irregular microarchitecture images suspected to malignancy were obtained from the metastases. In the porcine model, observation of abdominal organs was feasible without any organ injury in the laparoscopic procedures. The dosage of 1 {\%} fluorescein (3 ml/body) was appropriate in observing intra-abdominal organs, and each intra-abdominal organ was clearly observed with the same imaging quality we obtained in mice model. Conclusion: The pCLE was feasible and safe and potentially useful for the diagnosis of the peritoneal metastasis in in vivo animal models.",
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T1 - A novel approach of optical biopsy using probe-based confocal laser endomicroscopy for peritoneal metastasis

AU - Hara, Hisashi

AU - Takahashi, Tsuyoshi

AU - Nakatsuka, Rie

AU - Higashi, Shigeyoshi

AU - Naka, Tetsuji

AU - Sumiyama, Kazuki

AU - Miyazaki, Yasuhiro

AU - Makino, Tomoki

AU - Kurokawa, Yukinori

AU - Yamasaki, Makoto

AU - Takiguchi, Shuji

AU - Mori, Masaki

AU - Doki, Yuichiro

AU - Nakajima, Kiyokazu

PY - 2016/8/1

Y1 - 2016/8/1

N2 - Background: In digestive cancers, it is mandatory to diagnose peritoneal metastasis prior to selecting therapy. Therefore, exploratory laparoscopy has gained wider clinical acceptance. In laparoscopy, the peritoneal metastasis is pathologically confirmed by excisional biopsy; however, there remain technical difficulties in performing precise diagnosis and adequate biopsy on small peritoneal lesions without damaging organs. We have focused on “optical biopsy” using probe-based confocal laser endomicroscopy (pCLE). The aims of this study were (1) to optimize current CLE system for real-time observation of peritoneal metastases and (2) to assess its potential usefulness as diagnostic modality in preclinical settings. Methods: To optimize condition and evaluate feasibility, we prepared peritoneal metastasis mice model with gastric cancer cell line (MKN-45). On Day 10 after seeding, the mice were laparotomized and performed pCLE observations with CellvizioLAB (LSU-F 400/488 nm, Mauna Kea Technologies, Paris, France). We evaluated two different CLE probes, three different dyes, and optimal interval time. The detected sites were excised and pathologically evaluated on its morphology. Next, the feasibility and safety were validated in porcine model for clinical usage. After injection of fluorescein, pCLE was applied for the observation of intra-abdominal organs. Result: A miniature probe-type pCLE system with 60 μm focal depth (UltraMini O) and 1 % fluorescein dye was chosen for good visualization in mice model. The irregular microarchitecture images suspected to malignancy were obtained from the metastases. In the porcine model, observation of abdominal organs was feasible without any organ injury in the laparoscopic procedures. The dosage of 1 % fluorescein (3 ml/body) was appropriate in observing intra-abdominal organs, and each intra-abdominal organ was clearly observed with the same imaging quality we obtained in mice model. Conclusion: The pCLE was feasible and safe and potentially useful for the diagnosis of the peritoneal metastasis in in vivo animal models.

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