A novel archaeal lineage in boiling hot springs around oyasukyo gorge (Akita, Japan)

Katsuhiro Asamatsu, Kai Yoshitake, Makoto Saito, Wipoo Prasitwuttisak, Junichiro Ishibashi, Akihi Tsutsumi, Nurul Asyifah Mustapha, Toshinari Maeda, Katsunori Yanagawa

Research output: Contribution to journalArticlepeer-review

Abstract

A novel deep-branching archaeal lineage was discovered at high-temperature hot springs around Oyasukyo Gorge in Akita Prefecture, Japan. Actively boiling hot spring water contained >1×104 microbes mL–1. The microbial community composition assessed by analyzing 16S rRNA gene amplicons revealed that the dominant bacterial phyla were Proteobacteria and Aquificae (>50% of the microbial composition) in samples collected in 2016 and 2019, respectively. Approximately 10% of the reads obtained in both years were not assigned to any taxonomy. The more detailed phylogenetic positions of the unassigned sequences identified using a clone library and phylogenetic tree showed that they formed a clade that was independent, distantly related to known phyla, and had low similarity (<82%) to all other sequences in available databases. The present results suggest that this novel archaeal phylum-level lineage thrives in boiling hot springs in Japan.

Original languageEnglish
Article numberME21048
JournalMicrobes and environments
Volume36
Issue number4
DOIs
Publication statusPublished - 2021

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

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