Currently marketed first-generation drug-eluting stents still have several limitations. These adverse effects may be caused by either non-specific anti-proliferative effects on endothelial cells due to the drug used or to non-bioabsorbable polymers. To overcome these limitations, we developed a novel electro deposit coating technology using cationic bioabsorbable polymeric nanoparticles (NP). We prepared fluorescent chemical (FITC) or plasmid encoding green fluorescence protein (GFP) -encapsulated poly-lacticglycolic acid copolymer NP, whose surface was positively charged, by the emulsion solvent diffusion method. We succeeded in producing an homogenous coated stent by cationic electro deposit coating technology. To verify intracellular delivery of NP, a piece of NP-coated stent strut was placed in a dish of vascular smooth muscle cells in culture. After 2 hours, NP were uptaken by almost all of the cells. We then implanted the NP-eluting stents in porcine coronary arteries. Interestingly, intense fluorescence was observed in the neointima and media 28 days after deployment of the FITC-eluting stents. No evidence of abnormal inflammation in response to the NP-eluting stents was noted. The present study demonstrates the potential usefulness of our polymeric NP-eluting stents, resulting in a high-powered intracellular drug/gene delivery system for atherosclerotic lesions.
|Number of pages||10|
|Journal||Japanese Journal of Interventional Cardiology|
|Publication status||Published - 2007|
All Science Journal Classification (ASJC) codes
- Radiology Nuclear Medicine and imaging
- Cardiology and Cardiovascular Medicine