A novel bioabsorbable polymeric nanoparticle drug or gene eluting stent developed using electro-deposit nanoparticle-coating technology

Kaku Nakano, Kensuke Egashira, Seigo Masuda, Kouta Funakoshi, Satoshi Kimura, Hiroyuki Tsujimoto, Kaori Hara, Yoshiaki Kawashima, Ryuji Tominaga, Kenji Sunagawa

Research output: Contribution to journalArticle

Abstract

Currently marketed first-generation drug-eluting stents still have several limitations. These adverse effects may be caused by either non-specific anti-proliferative effects on endothelial cells due to the drug used or to non-bioabsorbable polymers. To overcome these limitations, we developed a novel electro deposit coating technology using cationic bioabsorbable polymeric nanoparticles (NP). We prepared fluorescent chemical (FITC) or plasmid encoding green fluorescence protein (GFP) -encapsulated poly-lacticglycolic acid copolymer NP, whose surface was positively charged, by the emulsion solvent diffusion method. We succeeded in producing an homogenous coated stent by cationic electro deposit coating technology. To verify intracellular delivery of NP, a piece of NP-coated stent strut was placed in a dish of vascular smooth muscle cells in culture. After 2 hours, NP were uptaken by almost all of the cells. We then implanted the NP-eluting stents in porcine coronary arteries. Interestingly, intense fluorescence was observed in the neointima and media 28 days after deployment of the FITC-eluting stents. No evidence of abnormal inflammation in response to the NP-eluting stents was noted. The present study demonstrates the potential usefulness of our polymeric NP-eluting stents, resulting in a high-powered intracellular drug/gene delivery system for atherosclerotic lesions.

Original languageEnglish
Pages (from-to)201-210
Number of pages10
JournalJapanese Journal of Interventional Cardiology
Volume22
Issue number3
Publication statusPublished - Jul 6 2007

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Nanoparticles
Stents
Technology
Pharmaceutical Preparations
Genes
Fluorescein-5-isothiocyanate
Fluorescence
Neointima
Gene Transfer Techniques
Drug-Eluting Stents
Drug Delivery Systems
Emulsions
Vascular Smooth Muscle
Smooth Muscle Myocytes
Coronary Vessels
Polymers
Plasmids
Swine
Endothelial Cells
Cell Culture Techniques

All Science Journal Classification (ASJC) codes

  • Radiology Nuclear Medicine and imaging
  • Cardiology and Cardiovascular Medicine

Cite this

A novel bioabsorbable polymeric nanoparticle drug or gene eluting stent developed using electro-deposit nanoparticle-coating technology. / Nakano, Kaku; Egashira, Kensuke; Masuda, Seigo; Funakoshi, Kouta; Kimura, Satoshi; Tsujimoto, Hiroyuki; Hara, Kaori; Kawashima, Yoshiaki; Tominaga, Ryuji; Sunagawa, Kenji.

In: Japanese Journal of Interventional Cardiology, Vol. 22, No. 3, 06.07.2007, p. 201-210.

Research output: Contribution to journalArticle

Nakano, K, Egashira, K, Masuda, S, Funakoshi, K, Kimura, S, Tsujimoto, H, Hara, K, Kawashima, Y, Tominaga, R & Sunagawa, K 2007, 'A novel bioabsorbable polymeric nanoparticle drug or gene eluting stent developed using electro-deposit nanoparticle-coating technology', Japanese Journal of Interventional Cardiology, vol. 22, no. 3, pp. 201-210.
Nakano, Kaku ; Egashira, Kensuke ; Masuda, Seigo ; Funakoshi, Kouta ; Kimura, Satoshi ; Tsujimoto, Hiroyuki ; Hara, Kaori ; Kawashima, Yoshiaki ; Tominaga, Ryuji ; Sunagawa, Kenji. / A novel bioabsorbable polymeric nanoparticle drug or gene eluting stent developed using electro-deposit nanoparticle-coating technology. In: Japanese Journal of Interventional Cardiology. 2007 ; Vol. 22, No. 3. pp. 201-210.
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