A novel Cdc42-interacting domain of the yeast polarity establishment protein Bem1: Implications for modulation of mating pheromone signaling

Yoshihiro Yamaguchi, Kazuhisa Ota, Takashi Ito

Research output: Contribution to journalArticle

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Abstract

In Saccharomyces cerevisiae, the Rho-type small GTPase Cdc42 is activated by its guanine-nucleotide exchange factor Cdc24 to polarize the cell for budding and mating. A multidomain protein Bem1 interacts not only with Cdc42 but also with Cdc24 and the effectors of Cdc42, including the p21-activated kinase Ste20, to function as a scaffold for cell polarity establishment. Although Bem1 interacts with Cdc24 and Ste20 via its PB1 and the second SH3 domains (SH3b), respectively, it is unclear how Bem1 binds Cdc42. Here we show that a region comprising the SH3b and its C-terminal flanking segment termed CI (SH3b-CI) directly interacts with Cdc42. A dual-bait reverse two-hybrid approach revealed that the CI is critical to the interaction: N253D substitution in the CI abolishes the binding of the SH3b-CI to Cdc42 but not to the proline-rich region of Ste20, whereas W192K substitution in the SH3b has the opposite effect. Nevertheless, the SH3b-CI interacts with Ste20 proline-rich region and Cdc42 in a mutually exclusive manner. The N253D substitution renders cellular growth temperature-sensitive and suppresses mating. The W192K-induced mating defect is exacerbated by the N253D substitution and suppressed by increasing the dosage of Ste20 provided that the CI is intact. Intriguingly, Cdc42 can mediate an indirect interaction of the SH3b-CI to the CRIB domain of Ste20. These results suggest that the SH3b and the CI collaborate in tethering of Ste20 to Bem1 to ensure efficient mating pheromone signaling.

Original languageEnglish
Pages (from-to)29-38
Number of pages10
JournalJournal of Biological Chemistry
Volume282
Issue number1
DOIs
Publication statusPublished - Jan 5 2007
Externally publishedYes

Fingerprint

Pheromones
Proline
Yeast
Substitution reactions
Yeasts
Modulation
p21-Activated Kinases
Guanine Nucleotide Exchange Factors
rho GTP-Binding Proteins
Cell Polarity
src Homology Domains
Monomeric GTP-Binding Proteins
Saccharomyces cerevisiae
Proteins
Temperature
Growth temperature
Growth
Scaffolds
Defects

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

A novel Cdc42-interacting domain of the yeast polarity establishment protein Bem1 : Implications for modulation of mating pheromone signaling. / Yamaguchi, Yoshihiro; Ota, Kazuhisa; Ito, Takashi.

In: Journal of Biological Chemistry, Vol. 282, No. 1, 05.01.2007, p. 29-38.

Research output: Contribution to journalArticle

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