A Novel Cell Fusion Method for Direct Cytoplasmic Transfer Using a Microfluidic Device

Ken Ichi Wada, Kazuo Hosokawa, Yoshihiro Ito, Mizuo Maeda

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

This chapter describes a novel cell fusion method for direct cytoplasmic transfer between live single cells. In order to realize this, we developed a microfluidic device which can make single cells pair at the position of microslit. Cell fusion was induced between the paired cells through a microslit by Sendai virus envelope (HVJ-E) to make strictured cytoplasmic connection. In the fused cell pairs, the nuclei in spread shape cells did not pass through the microslit. On the other hand, the nuclei in round shape cells migrated to the fusion partner leading to nuclear mixing. Supplementation with Y-27632, a ROCK inhibitor, prevented nuclear mixing and enhanced cell fusion rate. Mitochondria were transferred through the microslit under a condition of with or without Y-27632 supplementation. These data suggest that our cell fusion method using a newly developed microfluidic device can perform direct cytoplasmic transfer between live single cells.

Original languageEnglish
Title of host publicationACS Symposium Series
EditorsYoshihiro Ito, Xuesi Chen, Inn-Kyu Kang
PublisherAmerican Chemical Society
Pages227-236
Number of pages10
DOIs
Publication statusPublished - 2017
Externally publishedYes

Publication series

NameACS Symposium Series
Volume1253
ISSN (Print)0097-6156
ISSN (Electronic)1947-5918

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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