A novel cell layer without corticosteroid-synthesizing enzymes in rat adrenal cortex

Histochemical detection and possible physiological role

Fumiko Mitani, Hiroshi Suzuki, Jun Ichi Hata, Tadashi Ogishima, Hideo Shimada, Yuzuru Ishimura

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

A stratum of cells that did not contain both aldosterone synthase cytochrome P450 (cytochrome P450aldo) and cytochrome P45011β was found immunohistochemically between the zona glomerulosa and the zona fasciculata of the rat adrenal cortex. As cytochromes P450aldo and P45011β are the enzymes responsible for the biosynthesis of aldosterone and corticosterone, respectively, the cells there are considered to be incapable of synthesizing both aldosterone and corticosterone. Furthermore, the cells are regarded as inert in producing adrenal androgens, because rat adrenal cortex is known to lack steroid 17α-hydroxylase. Thus, the stratum is composed of cells that do not synthesize any of the major corticosteroids in significant quantities. It was 5-10 cells thick under normal feeding conditions, but diminished to 4-5 cells thick when animals were maintained under Na restriction, which is known to stimulate the secretion of angiotensin-II. When the distribution of 5- bromo-2'-deoxyuridine-labeled nuclei in the adrenocortex from BrdU administered rats was examined, the stained nuclei were concentrated in and around the cell stratum. The pulse-chase experiments showed that the labeled cells migrated out of this layer and into the zonae fasciculata-reticularis. On the basis of these findings, we suggest that the newly discovered cell layer is the progenitor cell zone of the rat adrenal cortex.

Original languageEnglish
Pages (from-to)431-438
Number of pages8
JournalEndocrinology
Volume135
Issue number1
DOIs
Publication statusPublished - Jan 1 1994

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Adrenal Cortex
Adrenal Cortex Hormones
Enzymes
Cytochrome P-450 CYP11B2
Zona Fasciculata
Bromodeoxyuridine
Corticosterone
Aldosterone
Zona Reticularis
Steroid 17-alpha-Hydroxylase
Zona Glomerulosa
Cytochromes
Angiotensin II
Cytochrome P-450 Enzyme System
Androgens
Stem Cells

All Science Journal Classification (ASJC) codes

  • Endocrinology

Cite this

A novel cell layer without corticosteroid-synthesizing enzymes in rat adrenal cortex : Histochemical detection and possible physiological role. / Mitani, Fumiko; Suzuki, Hiroshi; Hata, Jun Ichi; Ogishima, Tadashi; Shimada, Hideo; Ishimura, Yuzuru.

In: Endocrinology, Vol. 135, No. 1, 01.01.1994, p. 431-438.

Research output: Contribution to journalArticle

Mitani, Fumiko ; Suzuki, Hiroshi ; Hata, Jun Ichi ; Ogishima, Tadashi ; Shimada, Hideo ; Ishimura, Yuzuru. / A novel cell layer without corticosteroid-synthesizing enzymes in rat adrenal cortex : Histochemical detection and possible physiological role. In: Endocrinology. 1994 ; Vol. 135, No. 1. pp. 431-438.
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