A novel cytochrome P450 is implicated in brassinosteroid biosynthesis via the characterization of a rice dwarf mutant, dwarf11, with reduced seed length

Sumiyo Tanabe, Motoyuki Ashikari, Shozo Fujioka, Suguru Takatsuto, Shigeo Yoshida, Masahiro Yano, Atsushi Yoshimura, Hidemi Kitano, Makoto Matsuoka, Yukiko Fujisawa, Hisaharu Kato, Yukimoto Iwasaki

Research output: Contribution to journalArticle

271 Citations (Scopus)

Abstract

We have characterized a rice (Oryza sativa) dwarf mutant, dwarf11 (d11), that bears seeds of reduced length. To understand the mechanism by which seed length is regulated, the D11 gene was isolated by a map-based cloning method. The gene was found to encode a novel cytochrome P450 (CYP724B1), which showed homology to enzymes involved in brassinosteroid (BR) biosynthesis. The dwarf phenotype of d11 mutants was restored by the application of the brassinolide (BL). Compared with wild-type plants, the aberrant D11 mRNA accumulated at higher levels in d11 mutants and was dramatically reduced by treatment with BL, implying that the gene is feedback-regulated by BL. Precise determination of the defective step(s) in BR synthesis in d11 mutants proved intractable because of tissue specificity and the complex control of BR accumulation in plants. However, 6-deoxotyphasterol (6-DeoxoTY) and typhasterol (TY), but not any upstream intermediates before these compounds, effectively restored BR response in d11 mutants in a lamina joint bending assay. Multiple lines of evidence together suggest that the D11/CYP724B1 gene plays a role in BR synthesis and may be involved in the supply of 6-DeoxoTY and TY in the BR biosynthesis network in rice.

Original languageEnglish
Pages (from-to)776-790
Number of pages15
JournalPlant Cell
Volume17
Issue number3
DOIs
Publication statusPublished - Dec 1 2005

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Brassinosteroids
brassinosteroids
cytochrome P-450
Cytochrome P-450 Enzyme System
Seeds
biosynthesis
brassinolide
rice
mutants
seeds
Genes
genes
Organ Specificity
synthesis
laminae (animals)
Oryza
Organism Cloning
molecular cloning
Oryza sativa
Joints

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

A novel cytochrome P450 is implicated in brassinosteroid biosynthesis via the characterization of a rice dwarf mutant, dwarf11, with reduced seed length. / Tanabe, Sumiyo; Ashikari, Motoyuki; Fujioka, Shozo; Takatsuto, Suguru; Yoshida, Shigeo; Yano, Masahiro; Yoshimura, Atsushi; Kitano, Hidemi; Matsuoka, Makoto; Fujisawa, Yukiko; Kato, Hisaharu; Iwasaki, Yukimoto.

In: Plant Cell, Vol. 17, No. 3, 01.12.2005, p. 776-790.

Research output: Contribution to journalArticle

Tanabe, S, Ashikari, M, Fujioka, S, Takatsuto, S, Yoshida, S, Yano, M, Yoshimura, A, Kitano, H, Matsuoka, M, Fujisawa, Y, Kato, H & Iwasaki, Y 2005, 'A novel cytochrome P450 is implicated in brassinosteroid biosynthesis via the characterization of a rice dwarf mutant, dwarf11, with reduced seed length', Plant Cell, vol. 17, no. 3, pp. 776-790. https://doi.org/10.1105/tpc.104.024950
Tanabe, Sumiyo ; Ashikari, Motoyuki ; Fujioka, Shozo ; Takatsuto, Suguru ; Yoshida, Shigeo ; Yano, Masahiro ; Yoshimura, Atsushi ; Kitano, Hidemi ; Matsuoka, Makoto ; Fujisawa, Yukiko ; Kato, Hisaharu ; Iwasaki, Yukimoto. / A novel cytochrome P450 is implicated in brassinosteroid biosynthesis via the characterization of a rice dwarf mutant, dwarf11, with reduced seed length. In: Plant Cell. 2005 ; Vol. 17, No. 3. pp. 776-790.
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