A novel cytoplasmic GTPase XAB1 interacts with DNA repair protein XPA

Masahiko Nitta, Masafumi Saijo, Naohiko Kodo, Toshiro Matsuda, Yoshimichi Nakatsu, Hiroshi Tamai, Kiyoji Tanaka

Research output: Contribution to journalArticle

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Abstract

The xeroderma pigmentosum group A protein (XPA) plays a central role in nucleotide excision repair (NER). To identify proteins that bind to XPA, we screened a HeLa cDNA library using the yeast two-hybrid system. Here we report a novel cytoplasmic GTP-binding protein, designated XPA binding protein 1 (XAB1). The deduced amino acid sequence of XAB1 consisted of 374 residues with a molecular weight of 41 kDa and an isoelectric point of 4.65. Sequence analysis revealed that XAB1 has four sequence motifs G1-G4 of the GTP-binding protein family in the N-terminal half. XAB1 also contains an acidic region in the C-terminal portion. Northern blot analysis showed that XAB1 mRNA is expressed ubiquitously, and immunofluorescence analysis revealed that XAB1 is localized mainly in the cytoplasm. Consistent with the GTP-binding motif, purified recombinant XAB1 protein has intrinsic GTPase activity. Using the yeast two-hybrid system, we elucidated that XAB1 binds to the N-terminal region of XPA. The deletion of five amino acids, residues 30-34 of XPA, required for nuclear localization of XPA abolished the interaction with XAB1. These results suggest that XAB1 is a novel cytoplasmic GTPase involved in nuclear localization of XPA.

Original languageEnglish
Pages (from-to)4212-4218
Number of pages7
JournalNucleic acids research
Volume28
Issue number21
Publication statusPublished - Nov 1 2000

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GTP Phosphohydrolases
DNA Repair
Carrier Proteins
Proteins
Two-Hybrid System Techniques
GTP-Binding Proteins
Xeroderma Pigmentosum Group A Protein
Isoelectric Point
Guanosine Triphosphate
Gene Library
Northern Blotting
Fluorescent Antibody Technique
Sequence Analysis
Amino Acid Sequence
Cytoplasm
Molecular Weight
Amino Acids
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Nitta, M., Saijo, M., Kodo, N., Matsuda, T., Nakatsu, Y., Tamai, H., & Tanaka, K. (2000). A novel cytoplasmic GTPase XAB1 interacts with DNA repair protein XPA. Nucleic acids research, 28(21), 4212-4218.

A novel cytoplasmic GTPase XAB1 interacts with DNA repair protein XPA. / Nitta, Masahiko; Saijo, Masafumi; Kodo, Naohiko; Matsuda, Toshiro; Nakatsu, Yoshimichi; Tamai, Hiroshi; Tanaka, Kiyoji.

In: Nucleic acids research, Vol. 28, No. 21, 01.11.2000, p. 4212-4218.

Research output: Contribution to journalArticle

Nitta, M, Saijo, M, Kodo, N, Matsuda, T, Nakatsu, Y, Tamai, H & Tanaka, K 2000, 'A novel cytoplasmic GTPase XAB1 interacts with DNA repair protein XPA', Nucleic acids research, vol. 28, no. 21, pp. 4212-4218.
Nitta M, Saijo M, Kodo N, Matsuda T, Nakatsu Y, Tamai H et al. A novel cytoplasmic GTPase XAB1 interacts with DNA repair protein XPA. Nucleic acids research. 2000 Nov 1;28(21):4212-4218.
Nitta, Masahiko ; Saijo, Masafumi ; Kodo, Naohiko ; Matsuda, Toshiro ; Nakatsu, Yoshimichi ; Tamai, Hiroshi ; Tanaka, Kiyoji. / A novel cytoplasmic GTPase XAB1 interacts with DNA repair protein XPA. In: Nucleic acids research. 2000 ; Vol. 28, No. 21. pp. 4212-4218.
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