A novel DNA polymerase family found in Archaea

Yoshizumi Ishino, K. Komori, I. K.O. Cann, Y. Koga

Research output: Contribution to journalReview article

81 Citations (Scopus)

Abstract

One of the most puzzling results from the complete genome sequence of the methanogenic archaeon Methanococcus jannaschii was that the organism may have only one DNA polymerase gene. This is because no other DNA polymerase- like open reading frames (ORFs) were found besides one ORF having the typical α-like DNA polymerase (family B). Recently, we identified the genes of DNA polymerase II (the second DNA polymerase) from the hyperthermophilic archaeon Pyrococcus furiosus, which has also at least one α-like DNA polymerase (T. Uemori, Y. Sato, I. Kato, H. Doi, and Y. Ishino, Genes Cells 2:499-512, 1997). The genes in M. jannaschii encoding the proteins that are homologous to the DNA polymerase II of P. furiosus have been located and cloned. The gene products of M. jannaschii expressed in Escherichia coli had both DNA polymerizing and 3'→5' exonuclease activities. We propose here a novel DNA polymerase family which is entirely different from other hitherto-described DNA polymerases.

Original languageEnglish
Pages (from-to)2232-2236
Number of pages5
JournalJournal of bacteriology
Volume180
Issue number8
Publication statusPublished - May 21 1998
Externally publishedYes

Fingerprint

Archaea
DNA-Directed DNA Polymerase
Methanocaldococcus
DNA Polymerase II
Pyrococcus furiosus
spleen exonuclease
Genes
Open Reading Frames
Exodeoxyribonucleases
Phosphodiesterase I
Genome
Escherichia coli
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Ishino, Y., Komori, K., Cann, I. K. O., & Koga, Y. (1998). A novel DNA polymerase family found in Archaea. Journal of bacteriology, 180(8), 2232-2236.

A novel DNA polymerase family found in Archaea. / Ishino, Yoshizumi; Komori, K.; Cann, I. K.O.; Koga, Y.

In: Journal of bacteriology, Vol. 180, No. 8, 21.05.1998, p. 2232-2236.

Research output: Contribution to journalReview article

Ishino, Y, Komori, K, Cann, IKO & Koga, Y 1998, 'A novel DNA polymerase family found in Archaea', Journal of bacteriology, vol. 180, no. 8, pp. 2232-2236.
Ishino Y, Komori K, Cann IKO, Koga Y. A novel DNA polymerase family found in Archaea. Journal of bacteriology. 1998 May 21;180(8):2232-2236.
Ishino, Yoshizumi ; Komori, K. ; Cann, I. K.O. ; Koga, Y. / A novel DNA polymerase family found in Archaea. In: Journal of bacteriology. 1998 ; Vol. 180, No. 8. pp. 2232-2236.
@article{ade62b3681ec4a1a8b672673c0b88a6e,
title = "A novel DNA polymerase family found in Archaea",
abstract = "One of the most puzzling results from the complete genome sequence of the methanogenic archaeon Methanococcus jannaschii was that the organism may have only one DNA polymerase gene. This is because no other DNA polymerase- like open reading frames (ORFs) were found besides one ORF having the typical α-like DNA polymerase (family B). Recently, we identified the genes of DNA polymerase II (the second DNA polymerase) from the hyperthermophilic archaeon Pyrococcus furiosus, which has also at least one α-like DNA polymerase (T. Uemori, Y. Sato, I. Kato, H. Doi, and Y. Ishino, Genes Cells 2:499-512, 1997). The genes in M. jannaschii encoding the proteins that are homologous to the DNA polymerase II of P. furiosus have been located and cloned. The gene products of M. jannaschii expressed in Escherichia coli had both DNA polymerizing and 3'→5' exonuclease activities. We propose here a novel DNA polymerase family which is entirely different from other hitherto-described DNA polymerases.",
author = "Yoshizumi Ishino and K. Komori and Cann, {I. K.O.} and Y. Koga",
year = "1998",
month = "5",
day = "21",
language = "English",
volume = "180",
pages = "2232--2236",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "8",

}

TY - JOUR

T1 - A novel DNA polymerase family found in Archaea

AU - Ishino, Yoshizumi

AU - Komori, K.

AU - Cann, I. K.O.

AU - Koga, Y.

PY - 1998/5/21

Y1 - 1998/5/21

N2 - One of the most puzzling results from the complete genome sequence of the methanogenic archaeon Methanococcus jannaschii was that the organism may have only one DNA polymerase gene. This is because no other DNA polymerase- like open reading frames (ORFs) were found besides one ORF having the typical α-like DNA polymerase (family B). Recently, we identified the genes of DNA polymerase II (the second DNA polymerase) from the hyperthermophilic archaeon Pyrococcus furiosus, which has also at least one α-like DNA polymerase (T. Uemori, Y. Sato, I. Kato, H. Doi, and Y. Ishino, Genes Cells 2:499-512, 1997). The genes in M. jannaschii encoding the proteins that are homologous to the DNA polymerase II of P. furiosus have been located and cloned. The gene products of M. jannaschii expressed in Escherichia coli had both DNA polymerizing and 3'→5' exonuclease activities. We propose here a novel DNA polymerase family which is entirely different from other hitherto-described DNA polymerases.

AB - One of the most puzzling results from the complete genome sequence of the methanogenic archaeon Methanococcus jannaschii was that the organism may have only one DNA polymerase gene. This is because no other DNA polymerase- like open reading frames (ORFs) were found besides one ORF having the typical α-like DNA polymerase (family B). Recently, we identified the genes of DNA polymerase II (the second DNA polymerase) from the hyperthermophilic archaeon Pyrococcus furiosus, which has also at least one α-like DNA polymerase (T. Uemori, Y. Sato, I. Kato, H. Doi, and Y. Ishino, Genes Cells 2:499-512, 1997). The genes in M. jannaschii encoding the proteins that are homologous to the DNA polymerase II of P. furiosus have been located and cloned. The gene products of M. jannaschii expressed in Escherichia coli had both DNA polymerizing and 3'→5' exonuclease activities. We propose here a novel DNA polymerase family which is entirely different from other hitherto-described DNA polymerases.

UR - http://www.scopus.com/inward/record.url?scp=0031922356&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031922356&partnerID=8YFLogxK

M3 - Review article

C2 - 9555910

AN - SCOPUS:0031922356

VL - 180

SP - 2232

EP - 2236

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 8

ER -