A novel dnaK operon from Porphyromonas gingivalis

Akihiro Yoshida, Yoshio Nakano, Yoshihisa Yamashita, Takahiko Oho, Yukie Shibata, Masamichi Ohishi, Toshihiko Koga

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The nucleotide sequence of the dnaK operon cloned from Porphyromonas gingivalis revealed that the operon does not contain homologues of either dnaJ or grpE. However, there were two genes which encode small heat shock proteins immediately downstream from the dnaK and they were transcribed together with dnaK as one unit. The ATPase activity of the P. gingivalis DnaK was synergistically stimulated up to 40-fold in the simultaneous presence of Escherichia coli DnaJ and GrpE. These results suggest that the DnaK homologue of P. gingivalis, with its unique genetic structure and evolutionary features, works as a member of the DnaK chaperone system. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)287-291
Number of pages5
JournalFEBS Letters
Volume446
Issue number2-3
DOIs
Publication statusPublished - Mar 12 1999

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Small Heat-Shock Proteins
Porphyromonas gingivalis
Operon
Escherichia coli
Adenosine Triphosphatases
Nucleotides
Genes
Genetic Structures

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A novel dnaK operon from Porphyromonas gingivalis. / Yoshida, Akihiro; Nakano, Yoshio; Yamashita, Yoshihisa; Oho, Takahiko; Shibata, Yukie; Ohishi, Masamichi; Koga, Toshihiko.

In: FEBS Letters, Vol. 446, No. 2-3, 12.03.1999, p. 287-291.

Research output: Contribution to journalArticle

Yoshida, A, Nakano, Y, Yamashita, Y, Oho, T, Shibata, Y, Ohishi, M & Koga, T 1999, 'A novel dnaK operon from Porphyromonas gingivalis', FEBS Letters, vol. 446, no. 2-3, pp. 287-291. https://doi.org/10.1016/S0014-5793(99)00237-9
Yoshida, Akihiro ; Nakano, Yoshio ; Yamashita, Yoshihisa ; Oho, Takahiko ; Shibata, Yukie ; Ohishi, Masamichi ; Koga, Toshihiko. / A novel dnaK operon from Porphyromonas gingivalis. In: FEBS Letters. 1999 ; Vol. 446, No. 2-3. pp. 287-291.
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