A novel double-coating carrier produced by solid-in-oil and solid-in-water nanodispersion technology for delivery of genes and proteins into cells

Yoshiro Tahara, Takeshi Kaneko, Riki Toita, Chiharu Yoshiyama, Takuya Kitaoka, Takuro Niidome, Yoshiki Katayama, Noriho Kamiya, Masahiro Goto

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

A novel intracellular delivery method both for genes and proteins is one of the most coveted systems in the drug delivery field. In the present study, we developed a double-coating carrier loaded with gene and protein produced by solid-in-oil and solid-in-water nanodispersion technology. The double-coating carriers did not require electrostatic interactions during the preparation so were able to encapsulate plasmid DNA, ovalbumin (pI 4.5), horseradish peroxidase (pI 7.2), and cytochrome-c (pI 10.5) in a consistent manner. The carriers had practical encapsulation efficiencies and release profiles for genes and proteins. Furthermore, effective gene expression and cellular uptakes of both anionic and cationic proteins were achieved by modification of carriers with functional molecules. These findings indicate that the double-coating carrier has high potential for cellular delivery of various drugs and is a novel, superior method for both gene and protein delivery into cells.

Original languageEnglish
Pages (from-to)713-721
Number of pages9
JournalJournal of Controlled Release
Volume161
Issue number3
DOIs
Publication statusPublished - Aug 10 2012

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

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