A novel double-coating carrier produced by solid-in-oil and solid-in-water nanodispersion technology for delivery of genes and proteins into cells

Yoshiro Tahara, Takeshi Kaneko, Riki Toita, Chiharu Yoshiyama, Takuya Kitaoka, Takuro Niidome, Yoshiki Katayama, Noriho Kamiya, Masahiro Goto

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A novel intracellular delivery method both for genes and proteins is one of the most coveted systems in the drug delivery field. In the present study, we developed a double-coating carrier loaded with gene and protein produced by solid-in-oil and solid-in-water nanodispersion technology. The double-coating carriers did not require electrostatic interactions during the preparation so were able to encapsulate plasmid DNA, ovalbumin (pI 4.5), horseradish peroxidase (pI 7.2), and cytochrome-c (pI 10.5) in a consistent manner. The carriers had practical encapsulation efficiencies and release profiles for genes and proteins. Furthermore, effective gene expression and cellular uptakes of both anionic and cationic proteins were achieved by modification of carriers with functional molecules. These findings indicate that the double-coating carrier has high potential for cellular delivery of various drugs and is a novel, superior method for both gene and protein delivery into cells.

Original languageEnglish
Pages (from-to)713-721
Number of pages9
JournalJournal of Controlled Release
Volume161
Issue number3
DOIs
Publication statusPublished - Aug 10 2012

Fingerprint

Oils
Technology
Water
Proteins
Ovalbumin
Horseradish Peroxidase
Drug Delivery Systems
Cytochromes c
Static Electricity
Plasmids
Gene Expression
DNA
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this

A novel double-coating carrier produced by solid-in-oil and solid-in-water nanodispersion technology for delivery of genes and proteins into cells. / Tahara, Yoshiro; Kaneko, Takeshi; Toita, Riki; Yoshiyama, Chiharu; Kitaoka, Takuya; Niidome, Takuro; Katayama, Yoshiki; Kamiya, Noriho; Goto, Masahiro.

In: Journal of Controlled Release, Vol. 161, No. 3, 10.08.2012, p. 713-721.

Research output: Contribution to journalArticle

@article{d945b8b54aca4e1ea475e97177144cec,
title = "A novel double-coating carrier produced by solid-in-oil and solid-in-water nanodispersion technology for delivery of genes and proteins into cells",
abstract = "A novel intracellular delivery method both for genes and proteins is one of the most coveted systems in the drug delivery field. In the present study, we developed a double-coating carrier loaded with gene and protein produced by solid-in-oil and solid-in-water nanodispersion technology. The double-coating carriers did not require electrostatic interactions during the preparation so were able to encapsulate plasmid DNA, ovalbumin (pI 4.5), horseradish peroxidase (pI 7.2), and cytochrome-c (pI 10.5) in a consistent manner. The carriers had practical encapsulation efficiencies and release profiles for genes and proteins. Furthermore, effective gene expression and cellular uptakes of both anionic and cationic proteins were achieved by modification of carriers with functional molecules. These findings indicate that the double-coating carrier has high potential for cellular delivery of various drugs and is a novel, superior method for both gene and protein delivery into cells.",
author = "Yoshiro Tahara and Takeshi Kaneko and Riki Toita and Chiharu Yoshiyama and Takuya Kitaoka and Takuro Niidome and Yoshiki Katayama and Noriho Kamiya and Masahiro Goto",
year = "2012",
month = "8",
day = "10",
doi = "10.1016/j.jconrel.2012.05.001",
language = "English",
volume = "161",
pages = "713--721",
journal = "Journal of Controlled Release",
issn = "0168-3659",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - A novel double-coating carrier produced by solid-in-oil and solid-in-water nanodispersion technology for delivery of genes and proteins into cells

AU - Tahara, Yoshiro

AU - Kaneko, Takeshi

AU - Toita, Riki

AU - Yoshiyama, Chiharu

AU - Kitaoka, Takuya

AU - Niidome, Takuro

AU - Katayama, Yoshiki

AU - Kamiya, Noriho

AU - Goto, Masahiro

PY - 2012/8/10

Y1 - 2012/8/10

N2 - A novel intracellular delivery method both for genes and proteins is one of the most coveted systems in the drug delivery field. In the present study, we developed a double-coating carrier loaded with gene and protein produced by solid-in-oil and solid-in-water nanodispersion technology. The double-coating carriers did not require electrostatic interactions during the preparation so were able to encapsulate plasmid DNA, ovalbumin (pI 4.5), horseradish peroxidase (pI 7.2), and cytochrome-c (pI 10.5) in a consistent manner. The carriers had practical encapsulation efficiencies and release profiles for genes and proteins. Furthermore, effective gene expression and cellular uptakes of both anionic and cationic proteins were achieved by modification of carriers with functional molecules. These findings indicate that the double-coating carrier has high potential for cellular delivery of various drugs and is a novel, superior method for both gene and protein delivery into cells.

AB - A novel intracellular delivery method both for genes and proteins is one of the most coveted systems in the drug delivery field. In the present study, we developed a double-coating carrier loaded with gene and protein produced by solid-in-oil and solid-in-water nanodispersion technology. The double-coating carriers did not require electrostatic interactions during the preparation so were able to encapsulate plasmid DNA, ovalbumin (pI 4.5), horseradish peroxidase (pI 7.2), and cytochrome-c (pI 10.5) in a consistent manner. The carriers had practical encapsulation efficiencies and release profiles for genes and proteins. Furthermore, effective gene expression and cellular uptakes of both anionic and cationic proteins were achieved by modification of carriers with functional molecules. These findings indicate that the double-coating carrier has high potential for cellular delivery of various drugs and is a novel, superior method for both gene and protein delivery into cells.

UR - http://www.scopus.com/inward/record.url?scp=84864631918&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84864631918&partnerID=8YFLogxK

U2 - 10.1016/j.jconrel.2012.05.001

DO - 10.1016/j.jconrel.2012.05.001

M3 - Article

C2 - 22580226

AN - SCOPUS:84864631918

VL - 161

SP - 713

EP - 721

JO - Journal of Controlled Release

JF - Journal of Controlled Release

SN - 0168-3659

IS - 3

ER -