Abstract
A novel intracellular delivery method both for genes and proteins is one of the most coveted systems in the drug delivery field. In the present study, we developed a double-coating carrier loaded with gene and protein produced by solid-in-oil and solid-in-water nanodispersion technology. The double-coating carriers did not require electrostatic interactions during the preparation so were able to encapsulate plasmid DNA, ovalbumin (pI 4.5), horseradish peroxidase (pI 7.2), and cytochrome-c (pI 10.5) in a consistent manner. The carriers had practical encapsulation efficiencies and release profiles for genes and proteins. Furthermore, effective gene expression and cellular uptakes of both anionic and cationic proteins were achieved by modification of carriers with functional molecules. These findings indicate that the double-coating carrier has high potential for cellular delivery of various drugs and is a novel, superior method for both gene and protein delivery into cells.
| Original language | English |
|---|---|
| Pages (from-to) | 713-721 |
| Number of pages | 9 |
| Journal | Journal of Controlled Release |
| Volume | 161 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Aug 10 2012 |
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All Science Journal Classification (ASJC) codes
- Pharmaceutical Science
Cite this
A novel double-coating carrier produced by solid-in-oil and solid-in-water nanodispersion technology for delivery of genes and proteins into cells. / Tahara, Yoshiro; Kaneko, Takeshi; Toita, Riki; Yoshiyama, Chiharu; Kitaoka, Takuya; Niidome, Takuro; Katayama, Yoshiki; Kamiya, Noriho; Goto, Masahiro.
In: Journal of Controlled Release, Vol. 161, No. 3, 10.08.2012, p. 713-721.Research output: Contribution to journal › Article
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TY - JOUR
T1 - A novel double-coating carrier produced by solid-in-oil and solid-in-water nanodispersion technology for delivery of genes and proteins into cells
AU - Tahara, Yoshiro
AU - Kaneko, Takeshi
AU - Toita, Riki
AU - Yoshiyama, Chiharu
AU - Kitaoka, Takuya
AU - Niidome, Takuro
AU - Katayama, Yoshiki
AU - Kamiya, Noriho
AU - Goto, Masahiro
PY - 2012/8/10
Y1 - 2012/8/10
N2 - A novel intracellular delivery method both for genes and proteins is one of the most coveted systems in the drug delivery field. In the present study, we developed a double-coating carrier loaded with gene and protein produced by solid-in-oil and solid-in-water nanodispersion technology. The double-coating carriers did not require electrostatic interactions during the preparation so were able to encapsulate plasmid DNA, ovalbumin (pI 4.5), horseradish peroxidase (pI 7.2), and cytochrome-c (pI 10.5) in a consistent manner. The carriers had practical encapsulation efficiencies and release profiles for genes and proteins. Furthermore, effective gene expression and cellular uptakes of both anionic and cationic proteins were achieved by modification of carriers with functional molecules. These findings indicate that the double-coating carrier has high potential for cellular delivery of various drugs and is a novel, superior method for both gene and protein delivery into cells.
AB - A novel intracellular delivery method both for genes and proteins is one of the most coveted systems in the drug delivery field. In the present study, we developed a double-coating carrier loaded with gene and protein produced by solid-in-oil and solid-in-water nanodispersion technology. The double-coating carriers did not require electrostatic interactions during the preparation so were able to encapsulate plasmid DNA, ovalbumin (pI 4.5), horseradish peroxidase (pI 7.2), and cytochrome-c (pI 10.5) in a consistent manner. The carriers had practical encapsulation efficiencies and release profiles for genes and proteins. Furthermore, effective gene expression and cellular uptakes of both anionic and cationic proteins were achieved by modification of carriers with functional molecules. These findings indicate that the double-coating carrier has high potential for cellular delivery of various drugs and is a novel, superior method for both gene and protein delivery into cells.
UR - http://www.scopus.com/inward/record.url?scp=84864631918&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84864631918&partnerID=8YFLogxK
U2 - 10.1016/j.jconrel.2012.05.001
DO - 10.1016/j.jconrel.2012.05.001
M3 - Article
C2 - 22580226
AN - SCOPUS:84864631918
VL - 161
SP - 713
EP - 721
JO - Journal of Controlled Release
JF - Journal of Controlled Release
SN - 0168-3659
IS - 3
ER -