A novel endoscopic fluorescent clip visible with near-infrared imaging during laparoscopic surgery in a porcine model

Hiroshi Takeyama, Taishi Hata, Junichi Nishimura, Ryoji Nonaka, Mamoru Uemura, Naotsugu Haraguchi, Ichiro Takemasa, Tsunekazu Mizushima, Hirofumi Yamamoto, Yuichiro Doki, Masaki Mori

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: In gastrointestinal cancer surgery, particularly in early cancer, accurate tumor localization is important in order to determine the extent of resection. In laparoscopic surgery, because of the inability to palpate the lesion, the most prevalent method of localization is endoscopic tattooing. However, complicated maneuvering makes it difficult to control local dye spreading and dye leakage into the intraperitoneal cavity. A simpler, safe method is needed. In this study, we developed a novel method for applying fluorescence-coated endoscopic clips to visualize locations inside the colon during laparoscopic surgery. We tested the procedure in an in vivo porcine model and with ex vivo human colon tissues. Methods: Bovine serum albumin was conjugated to indocyanine green or the succinimidyl ester CF™ 790 to form a pasty mixture, which was used to coat the front ends of endoscopic clips. The fluorescence-coated clips were endoscopically placed on the mucosal surface of a porcine colon. Using an Olympus near-infrared laparoscopy system, we attempted to identify the fluorescent clips from the outer, serosal side of the porcine colon during laparoscopic surgery in vivo. The clips were also evaluated using ex vivo human colon tissues. Results: After placing two clips on the inner, mucosal surface of the porcine colon, we used near-infrared laparoscopy to view them from the outer, serosal surface of the colon in real time during in vivo laparoscopic surgery. We also identified the fluorescence-coated clips through human colon tissues in an ex vivo study. Conclusions: We developed a novel, fluorescence-coated clip that can be placed endoscopically for rapid, exact localization of colonic lesions. The clips were successfully visualized with near-infrared fluorescence imaging during laparoscopic surgery in an in vivo porcine model and in ex vivo human colon tissues.

Original languageEnglish
Pages (from-to)1984-1990
Number of pages7
JournalSurgical endoscopy
Volume28
Issue number6
DOIs
Publication statusPublished - Jan 1 2014

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Surgical Instruments
Laparoscopy
Colon
Swine
Fluorescence
Coloring Agents
Tattooing
Gastrointestinal Neoplasms
Indocyanine Green
Optical Imaging
Bovine Serum Albumin
Neoplasms
Esters

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

A novel endoscopic fluorescent clip visible with near-infrared imaging during laparoscopic surgery in a porcine model. / Takeyama, Hiroshi; Hata, Taishi; Nishimura, Junichi; Nonaka, Ryoji; Uemura, Mamoru; Haraguchi, Naotsugu; Takemasa, Ichiro; Mizushima, Tsunekazu; Yamamoto, Hirofumi; Doki, Yuichiro; Mori, Masaki.

In: Surgical endoscopy, Vol. 28, No. 6, 01.01.2014, p. 1984-1990.

Research output: Contribution to journalArticle

Takeyama, H, Hata, T, Nishimura, J, Nonaka, R, Uemura, M, Haraguchi, N, Takemasa, I, Mizushima, T, Yamamoto, H, Doki, Y & Mori, M 2014, 'A novel endoscopic fluorescent clip visible with near-infrared imaging during laparoscopic surgery in a porcine model', Surgical endoscopy, vol. 28, no. 6, pp. 1984-1990. https://doi.org/10.1007/s00464-014-3423-z
Takeyama, Hiroshi ; Hata, Taishi ; Nishimura, Junichi ; Nonaka, Ryoji ; Uemura, Mamoru ; Haraguchi, Naotsugu ; Takemasa, Ichiro ; Mizushima, Tsunekazu ; Yamamoto, Hirofumi ; Doki, Yuichiro ; Mori, Masaki. / A novel endoscopic fluorescent clip visible with near-infrared imaging during laparoscopic surgery in a porcine model. In: Surgical endoscopy. 2014 ; Vol. 28, No. 6. pp. 1984-1990.
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AU - Uemura, Mamoru

AU - Haraguchi, Naotsugu

AU - Takemasa, Ichiro

AU - Mizushima, Tsunekazu

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AU - Doki, Yuichiro

AU - Mori, Masaki

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N2 - Background: In gastrointestinal cancer surgery, particularly in early cancer, accurate tumor localization is important in order to determine the extent of resection. In laparoscopic surgery, because of the inability to palpate the lesion, the most prevalent method of localization is endoscopic tattooing. However, complicated maneuvering makes it difficult to control local dye spreading and dye leakage into the intraperitoneal cavity. A simpler, safe method is needed. In this study, we developed a novel method for applying fluorescence-coated endoscopic clips to visualize locations inside the colon during laparoscopic surgery. We tested the procedure in an in vivo porcine model and with ex vivo human colon tissues. Methods: Bovine serum albumin was conjugated to indocyanine green or the succinimidyl ester CF™ 790 to form a pasty mixture, which was used to coat the front ends of endoscopic clips. The fluorescence-coated clips were endoscopically placed on the mucosal surface of a porcine colon. Using an Olympus near-infrared laparoscopy system, we attempted to identify the fluorescent clips from the outer, serosal side of the porcine colon during laparoscopic surgery in vivo. The clips were also evaluated using ex vivo human colon tissues. Results: After placing two clips on the inner, mucosal surface of the porcine colon, we used near-infrared laparoscopy to view them from the outer, serosal surface of the colon in real time during in vivo laparoscopic surgery. We also identified the fluorescence-coated clips through human colon tissues in an ex vivo study. Conclusions: We developed a novel, fluorescence-coated clip that can be placed endoscopically for rapid, exact localization of colonic lesions. The clips were successfully visualized with near-infrared fluorescence imaging during laparoscopic surgery in an in vivo porcine model and in ex vivo human colon tissues.

AB - Background: In gastrointestinal cancer surgery, particularly in early cancer, accurate tumor localization is important in order to determine the extent of resection. In laparoscopic surgery, because of the inability to palpate the lesion, the most prevalent method of localization is endoscopic tattooing. However, complicated maneuvering makes it difficult to control local dye spreading and dye leakage into the intraperitoneal cavity. A simpler, safe method is needed. In this study, we developed a novel method for applying fluorescence-coated endoscopic clips to visualize locations inside the colon during laparoscopic surgery. We tested the procedure in an in vivo porcine model and with ex vivo human colon tissues. Methods: Bovine serum albumin was conjugated to indocyanine green or the succinimidyl ester CF™ 790 to form a pasty mixture, which was used to coat the front ends of endoscopic clips. The fluorescence-coated clips were endoscopically placed on the mucosal surface of a porcine colon. Using an Olympus near-infrared laparoscopy system, we attempted to identify the fluorescent clips from the outer, serosal side of the porcine colon during laparoscopic surgery in vivo. The clips were also evaluated using ex vivo human colon tissues. Results: After placing two clips on the inner, mucosal surface of the porcine colon, we used near-infrared laparoscopy to view them from the outer, serosal surface of the colon in real time during in vivo laparoscopic surgery. We also identified the fluorescence-coated clips through human colon tissues in an ex vivo study. Conclusions: We developed a novel, fluorescence-coated clip that can be placed endoscopically for rapid, exact localization of colonic lesions. The clips were successfully visualized with near-infrared fluorescence imaging during laparoscopic surgery in an in vivo porcine model and in ex vivo human colon tissues.

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