A novel enzymatic glucose biosensor and sensitive non-enzymatic hydrogen peroxide sensor based on graphene and cobalt oxide nanoparticles composite modified glassy carbon electrode

Chelladurai Karuppiah, Selvakumar Palanisamy, Shen Ming Chen, Vediyappan Veeramani, Prakash Periakaruppan

Research output: Contribution to journalArticlepeer-review

101 Citations (Scopus)

Abstract

In the present study, we have demonstrated the fabrication of novel enzymatic glucose biosensor using glucose oxidase (GOD) as a model enzyme which has been immobilized onto the graphene (GF) and cobalt oxide nanoparticles (Co3O4-NPs) composite modified electrode. The GF/Co 3O4-NPs composite was prepared by hydrothermal method and characterized by using scanning electron microscopy, X-ray diffraction and elemental analysis. The GOD immobilized GF/Co3O4-NPs modified electrode shows a well defined redox behaviour indicating the reversible proton and electron transfer reaction of GOD. A heterogeneous electron transfer rate constant (Ks) of immobilized GOD has been calculated to be 3.52 s-1 which is much higher than that of GOD immobilized GF supports. The fast electron transfer of GOD is attributed to the excellent biocompatibility of Co3O4-NPs and high conductivity of the GF. The fabricated glucose biosensor exhibits a wider linear response for glucose from 0.5 mM to 16.5 mM with the sensitivity of 13.52 μA mM-1 cm-2. In addition, a non-enzymatic H 2O2 sensor has been further developed using GF/Co 3O4-NPs composite modified electrode. The GF/Co 3O4-NPs composite electrode shows an excellent electrocatalytic activity towards H2O2 with the response time of <10 s. The H2O2 response at GF/Co 3O4-NPs composite modified electrode displays a linear response ranging from 0.2 to 211.5 μM with a limit of detection of 0.06 μM.

Original languageEnglish
Pages (from-to)450-456
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume196
DOIs
Publication statusPublished - Jun 2014
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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