A novel enzyme that cleaves the N-acyl linkage of ceramides in various glycosphingolipids as well as sphingomyelin to produce their lyso forms

Makoto Ito, T. Kurita, K. Kita

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

We describe a novel enzyme that hydrolyzes the N-acyl linkage between fatty acids and sphingosine bases in ceramides of various sphingolipids. The enzyme was purified about 300-fold with 5% recovery from the culture filtrate of a newly isolated bacterium (Pseudomonas sp. TK4) by ammonium sulfate precipitation followed by several steps of high performance liquid chromatography. The purified enzyme preparation was completely free of exoglycosidases, sphingomyelinase, and proteases, and showed a single protein band corresponding to a molecular mass of 52 kDa on SOS-polyacrylamide slab gel electrophoresis after staining with Coomassie Brilliant Blue. The enzyme shows quite wide specificity, i.e. it hydrolyzes both neutral and acidic glycosphingolipids, and simple glycosphingolipid cerebrosides to polysialogangliosides such as GQ1b. Furthermore the enzyme also hydrolyzes sphingomyelin to produce the respective lyso form. However, the enzyme shows hardly any activity on ceramides, indicating that it is completely different from the ceramidase (EC 3.5.1.23) reported previously. This enzyme, which is tentatively named sphingolipid ceramide N-deacylase, should greatly facilitate the further study of sphingolipids as well as lysosphingolipids.

Original languageEnglish
Pages (from-to)24370-24374
Number of pages5
JournalJournal of Biological Chemistry
Volume270
Issue number41
DOIs
Publication statusPublished - 1995

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Glycosphingolipids
Sphingomyelins
Ceramides
Enzymes
Sphingolipids
Acidic Glycosphingolipids
Ceramidases
Neutral Glycosphingolipids
Cerebrosides
Sphingomyelin Phosphodiesterase
Sphingosine
Glycoside Hydrolases
Ammonium Sulfate
High performance liquid chromatography
Molecular mass
Pseudomonas
Electrophoresis
Polyacrylamide Gel Electrophoresis
Bacteria
Peptide Hydrolases

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

A novel enzyme that cleaves the N-acyl linkage of ceramides in various glycosphingolipids as well as sphingomyelin to produce their lyso forms. / Ito, Makoto; Kurita, T.; Kita, K.

In: Journal of Biological Chemistry, Vol. 270, No. 41, 1995, p. 24370-24374.

Research output: Contribution to journalArticle

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