TY - JOUR
T1 - A novel function of CD82/KAI-1 on E-cadherin-mediated homophilic cellular adhesion of cancer cells
AU - Abe, Masakazu
AU - Sugiura, Tsuyoshi
AU - Takahashi, Miho
AU - Ishii, Kotaro
AU - Shimoda, Miyuki
AU - Shirasuna, Kanemitsu
N1 - Funding Information:
This work was supported by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (to T. Sugiura).
PY - 2008/8/8
Y1 - 2008/8/8
N2 - In this study, we analyzed the effect of the metastasis suppressor CD82/KAI-1, a member of the tetraspanin superfamily, on intercellular adhesion on cancer cells. The newly established invasion assay and the cell aggregation assay revealed that CD82 strengthens E-cadherin-mediated intercellular adhesion. Interestingly, ectopic expression of CD82 stabilized E-cadherin/β-catenin complex formation. Furthermore, CD82 reduced tyrosine phosphorylation of β-catenin on HGF stimulation. Taken together, CD82 may stabilize or strengthen E-cadherin-dependent intercellular adhesion by regulating β-catenin-mediated signal transduction on cancer cells, and consequently, prevent cancer cells from seceding from the primary tumor site.
AB - In this study, we analyzed the effect of the metastasis suppressor CD82/KAI-1, a member of the tetraspanin superfamily, on intercellular adhesion on cancer cells. The newly established invasion assay and the cell aggregation assay revealed that CD82 strengthens E-cadherin-mediated intercellular adhesion. Interestingly, ectopic expression of CD82 stabilized E-cadherin/β-catenin complex formation. Furthermore, CD82 reduced tyrosine phosphorylation of β-catenin on HGF stimulation. Taken together, CD82 may stabilize or strengthen E-cadherin-dependent intercellular adhesion by regulating β-catenin-mediated signal transduction on cancer cells, and consequently, prevent cancer cells from seceding from the primary tumor site.
UR - http://www.scopus.com/inward/record.url?scp=45049085596&partnerID=8YFLogxK
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U2 - 10.1016/j.canlet.2008.02.058
DO - 10.1016/j.canlet.2008.02.058
M3 - Article
C2 - 18395972
AN - SCOPUS:45049085596
SN - 0304-3835
VL - 266
SP - 163
EP - 170
JO - Cancer Letters
JF - Cancer Letters
IS - 2
ER -
