Abstract
Differential display screening for region-specific transcripts in rat brain revealed a novel striatum-specific transcript encoding an orphan G-protein-coupled receptor (GPCR) designated Strg/Gpr88 for striatum-specific GPCR. We isolated its homologues from human (HGMW-approved symbol GPR88) and mouse and mapped them to chromosomes 1p21.3 and 3G1, respectively. These loci are syntenic to each other, thereby suggesting their orthology. The predicted primary sequences of Strg/Gpr88 proteins are highly conserved between human and rodents and show the highest level of homology to receptors for biogenic amines. However, Strg/Gpr88 lacks some residues conserved in all known biogenic amine receptors and hence may represent a novel subtype of GPCR. Northern blot and in situ hybridization analyses revealed that Strg/Gpr88 transcripts are expressed almost exclusively in striatum in both human and rodents. Remarkable conservation in primary structure and a unique expression pattern may indicate a role for Strg/Gpr88 in the fundamental functions of striatum such as the control of motor behavior. (C) 2000 Academic Press.
| Original language | English |
|---|---|
| Pages (from-to) | 314-321 |
| Number of pages | 8 |
| Journal | Genomics |
| Volume | 69 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Nov 1 2000 |
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All Science Journal Classification (ASJC) codes
- Genetics
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A novel G-protein-coupled receptor gene expressed in striatum. / Mizushima, Kazuyuki; Miyamoto, Yasuhide; Tsukahara, Fujiko; Hirai, Momoki; Sakaki, Yoshiyuki; Ito, Takashi.
In: Genomics, Vol. 69, No. 3, 01.11.2000, p. 314-321.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A novel G-protein-coupled receptor gene expressed in striatum
AU - Mizushima, Kazuyuki
AU - Miyamoto, Yasuhide
AU - Tsukahara, Fujiko
AU - Hirai, Momoki
AU - Sakaki, Yoshiyuki
AU - Ito, Takashi
PY - 2000/11/1
Y1 - 2000/11/1
N2 - Differential display screening for region-specific transcripts in rat brain revealed a novel striatum-specific transcript encoding an orphan G-protein-coupled receptor (GPCR) designated Strg/Gpr88 for striatum-specific GPCR. We isolated its homologues from human (HGMW-approved symbol GPR88) and mouse and mapped them to chromosomes 1p21.3 and 3G1, respectively. These loci are syntenic to each other, thereby suggesting their orthology. The predicted primary sequences of Strg/Gpr88 proteins are highly conserved between human and rodents and show the highest level of homology to receptors for biogenic amines. However, Strg/Gpr88 lacks some residues conserved in all known biogenic amine receptors and hence may represent a novel subtype of GPCR. Northern blot and in situ hybridization analyses revealed that Strg/Gpr88 transcripts are expressed almost exclusively in striatum in both human and rodents. Remarkable conservation in primary structure and a unique expression pattern may indicate a role for Strg/Gpr88 in the fundamental functions of striatum such as the control of motor behavior. (C) 2000 Academic Press.
AB - Differential display screening for region-specific transcripts in rat brain revealed a novel striatum-specific transcript encoding an orphan G-protein-coupled receptor (GPCR) designated Strg/Gpr88 for striatum-specific GPCR. We isolated its homologues from human (HGMW-approved symbol GPR88) and mouse and mapped them to chromosomes 1p21.3 and 3G1, respectively. These loci are syntenic to each other, thereby suggesting their orthology. The predicted primary sequences of Strg/Gpr88 proteins are highly conserved between human and rodents and show the highest level of homology to receptors for biogenic amines. However, Strg/Gpr88 lacks some residues conserved in all known biogenic amine receptors and hence may represent a novel subtype of GPCR. Northern blot and in situ hybridization analyses revealed that Strg/Gpr88 transcripts are expressed almost exclusively in striatum in both human and rodents. Remarkable conservation in primary structure and a unique expression pattern may indicate a role for Strg/Gpr88 in the fundamental functions of striatum such as the control of motor behavior. (C) 2000 Academic Press.
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U2 - 10.1006/geno.2000.6340
DO - 10.1006/geno.2000.6340
M3 - Article
C2 - 11056049
AN - SCOPUS:0034331156
VL - 69
SP - 314
EP - 321
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 3
ER -