A novel glycosphingolipid-degrading enzyme cleaves of the linkage between the oligosaccharide and ceramide of neutral and acidic glycosphingolipids

Makoto Ito, T. Yamagata

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

A novel glycosphingolipid-degrading enzyme was found in the cultured supernatant of Rhodococcus sp. G-74-2. It was purified 34.7-fold from the supernatant with 32.2% recovery by ammonium sulfate precipitation followed by Sephadex G-100 chromatography. The enzyme was demonstrated capable of cleaving the linkage between the oligosaccharide and ceramide of various acidic and neutral glycosphingolipids, producing intact oligosaccharides and ceramides. However, it was noted to hardly make any attack on linkages between monosaccharides and ceramides (cerebrosides) or between oligosaccharides and diacylglycerol (glycoglycerolipids). The enzyme preparation was completely free from various exoglycosidases and proteases. Furthermore, it was found to degrade neither N-linked nor O-linked glycoproteins. This enzyme, which is tentatively called endoglycoceramidase, should greatly facilitate the study of glycosphingolipids.

Original languageEnglish
Pages (from-to)14278-14282
Number of pages5
JournalJournal of Biological Chemistry
Volume261
Issue number30
Publication statusPublished - 1986
Externally publishedYes

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Acidic Glycosphingolipids
Neutral Glycosphingolipids
Glycosphingolipids
Ceramides
Oligosaccharides
endoglycoceramidase
Enzymes
Cerebrosides
Rhodococcus
Monosaccharides
Glycoside Hydrolases
Diglycerides
Ammonium Sulfate
Chromatography
Glycoproteins
Peptide Hydrolases
Recovery

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

A novel glycosphingolipid-degrading enzyme cleaves of the linkage between the oligosaccharide and ceramide of neutral and acidic glycosphingolipids. / Ito, Makoto; Yamagata, T.

In: Journal of Biological Chemistry, Vol. 261, No. 30, 1986, p. 14278-14282.

Research output: Contribution to journalArticle

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