A novel mechanism for glucose side-chain formation in rhamnose-glucose polysaccharide synthesis

Kazuhisa Ozaki, Yukie Shibata, Yoshihisa Yamashita, Yoshio Nakano, Hiromasa Tsuda, Toshihiko Koga

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We have cloned two genes (rgpH and rgpI) that encode proteins for the formation of the glucose side-chains of the Streptococcus mutans rhamnose-glucose polysaccharide (RGP), which consists of a rhamnan backbone with glucose side-chains. The roles of rgpH and rgpI were evaluated in a rhamnan-synthesizing Escherichia coli. An E. coli strain that harbored rgpH reacted with antiserum directed against complete RGP, whereas the E. coli strain that carried rgpI did not react with this antiserum. Although E. coli:rgpH reacted strongly with rhamnan-specific antiserum, co-transformation of this strain with rgpI increased the number of glucose side-chains and decreased immunoreactivity with the rhamnan-specific antiserum significantly. These results suggest that two genes are involved in side-chain formation during S. mutans RGP synthesis in E. coli: one gene encodes a glucosyltransferase, and the other gene probably controls the frequency of branching. This is the first report to identify a gene that is involved in regulation of branching frequency in polysaccharide synthesis.

Original languageEnglish
Pages (from-to)159-163
Number of pages5
JournalFEBS Letters
Volume532
Issue number1-2
DOIs
Publication statusPublished - Dec 4 2002

Fingerprint

Rhamnose
Polysaccharides
Escherichia coli
Glucose
Genes
Immune Sera
Streptococcus mutans
Glucosyltransferases
rhamnan
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

A novel mechanism for glucose side-chain formation in rhamnose-glucose polysaccharide synthesis. / Ozaki, Kazuhisa; Shibata, Yukie; Yamashita, Yoshihisa; Nakano, Yoshio; Tsuda, Hiromasa; Koga, Toshihiko.

In: FEBS Letters, Vol. 532, No. 1-2, 04.12.2002, p. 159-163.

Research output: Contribution to journalArticle

Ozaki, Kazuhisa ; Shibata, Yukie ; Yamashita, Yoshihisa ; Nakano, Yoshio ; Tsuda, Hiromasa ; Koga, Toshihiko. / A novel mechanism for glucose side-chain formation in rhamnose-glucose polysaccharide synthesis. In: FEBS Letters. 2002 ; Vol. 532, No. 1-2. pp. 159-163.
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