TY - JOUR
T1 - A novel method for the assay of α-glucosidase inhibitory activity using a multi-channel oxygen sensor
AU - Matsumoto, Kiyoshi
AU - Takemata, Kayoko
AU - Takayama, Kiyofumi
AU - Abesundara, Kanthi J.M.
AU - Matsui, Toshiro
AU - Katayama, Hideo
PY - 2002/12/1
Y1 - 2002/12/1
N2 - The quantification of glucose by using a multi-channel dissolved oxygen (DO) meter (DOX96) with immobilized glucose oxidase (GOD) and mutarotase (MUT) was performed. An evaluation of the inhibitory activities for α-glucosidase (AGH) by modifying our batch-type pseudo-in vivo assay system [Oki et al.; Biol. Pharm. Bull., 2000, 232, 1084] was also performed using a DOX96. When 45 U/well GOD and 18.75 U/well MUT were immobilized on the surface of a gelatin membrane on the electrodes, the response shown by the decrease percent of DO (%) obtained with 8 electrode wells in the same row was linear with the glucose concentration up to 3.3 mM and a correlation coefficient larger than 0.9. To estimate the AGH inhibitory activity, AGH-immobilized Sepharose supports in the well of a silent screen plate were used. The IC50 values of acarbose and 1-deoxynojirimycin, a medicinal inhibitor for diabetes, were 0.70 ± 0.08 μM and 0.40 ± 0.13 μM, respectively, and coincided well with those by a pseudo-in vivo assay.
AB - The quantification of glucose by using a multi-channel dissolved oxygen (DO) meter (DOX96) with immobilized glucose oxidase (GOD) and mutarotase (MUT) was performed. An evaluation of the inhibitory activities for α-glucosidase (AGH) by modifying our batch-type pseudo-in vivo assay system [Oki et al.; Biol. Pharm. Bull., 2000, 232, 1084] was also performed using a DOX96. When 45 U/well GOD and 18.75 U/well MUT were immobilized on the surface of a gelatin membrane on the electrodes, the response shown by the decrease percent of DO (%) obtained with 8 electrode wells in the same row was linear with the glucose concentration up to 3.3 mM and a correlation coefficient larger than 0.9. To estimate the AGH inhibitory activity, AGH-immobilized Sepharose supports in the well of a silent screen plate were used. The IC50 values of acarbose and 1-deoxynojirimycin, a medicinal inhibitor for diabetes, were 0.70 ± 0.08 μM and 0.40 ± 0.13 μM, respectively, and coincided well with those by a pseudo-in vivo assay.
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U2 - 10.2116/analsci.18.1315
DO - 10.2116/analsci.18.1315
M3 - Article
C2 - 12502081
AN - SCOPUS:0036947867
SN - 0910-6340
VL - 18
SP - 1315
EP - 1319
JO - Analytical Sciences
JF - Analytical Sciences
IS - 12
ER -
