TY - JOUR
T1 - A novel neuropeptide, Hym-176, induces contraction of the ectodermal muscle in Hydra
AU - Yum, Seungshic
AU - Takahashi, Toshio
AU - Koizumi, Osamu
AU - Ariura, Yuki
AU - Kobayakawa, Yoshitaka
AU - Mohri, Shirou
AU - Fujisawa, Toshitaka
PY - 1998/7/30
Y1 - 1998/7/30
N2 - During the course of a systematic non-targeting screening of peptide signal molecules in Hydra, we identified a novel myoactive neuropeptide called Hym-176. The primary structure of Hym-176 was determined to be APFIFPGPKV amide. It specifically and reversibly induced contraction of the ectodermal muscle of the hydra body column in vivo. However, it had no effect on the ectodermal muscle of the tentacles. The structure-activity relationship analysis showed that the sequence of FIFPGPKV amide is a minimal requirement for the myoactivity. Removal of an amide group from the C-terminus completely abolished the activity. By using the antibody specific to Hym-176, the tissue localization of the peptide in hydra was determined immunohistochemically. The intense immunoreactivity was found in the peduncle nerve cells, indicating that Hym-176 is a neuropeptide.
AB - During the course of a systematic non-targeting screening of peptide signal molecules in Hydra, we identified a novel myoactive neuropeptide called Hym-176. The primary structure of Hym-176 was determined to be APFIFPGPKV amide. It specifically and reversibly induced contraction of the ectodermal muscle of the hydra body column in vivo. However, it had no effect on the ectodermal muscle of the tentacles. The structure-activity relationship analysis showed that the sequence of FIFPGPKV amide is a minimal requirement for the myoactivity. Removal of an amide group from the C-terminus completely abolished the activity. By using the antibody specific to Hym-176, the tissue localization of the peptide in hydra was determined immunohistochemically. The intense immunoreactivity was found in the peduncle nerve cells, indicating that Hym-176 is a neuropeptide.
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U2 - 10.1006/bbrc.1998.8831
DO - 10.1006/bbrc.1998.8831
M3 - Article
C2 - 9703970
AN - SCOPUS:0032581335
VL - 248
SP - 584
EP - 590
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -