A novel production process for optically pure l-lactic acid from kitchen refuse using a bacterial consortium at high temperatures

Yukihiro Tashiro, Hiroko Matsumoto, Hirokuni Miyamoto, Yuki Okugawa, Poudel Pramod, Hisashi Miyamoto, Kenji Sakai

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We investigated l-lactic acid production in static batch fermentation of kitchen refuse using a bacterial consortium from marine-animal-resource (MAR) composts at temperatures ranging from 30 to 65°C. At relatively low temperatures butyric acid accumulated, whereas at higher temperatures l-lactic acid was produced. In particular, fermentation at 50°C produced 34.5gL-1 l-lactic acid with 90% lactic acid selectivity and 100% optical purity. Denaturing gradient gel electrophoresis indicated that dominant bacteria present in the original MAR composts diminished rapidly and Bacillus coagulans strains became the dominant contributors to l-lactic acid production at 45, 50 and 55°C. This is the first report of the achievement of 100% optical purity of l-lactic acid using a bacterial consortium.

Original languageEnglish
Pages (from-to)672-681
Number of pages10
JournalBioresource Technology
Volume146
DOIs
Publication statusPublished - Jan 1 2013

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Kitchens
Lactic acid
refuse
Lactic Acid
acid
Fermentation
Temperature
Animals
compost
fermentation
Butyric acid
Butyric Acid
Bacilli
Electrophoresis
Bacteria
Gels
electrokinesis
gel
bacterium

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

A novel production process for optically pure l-lactic acid from kitchen refuse using a bacterial consortium at high temperatures. / Tashiro, Yukihiro; Matsumoto, Hiroko; Miyamoto, Hirokuni; Okugawa, Yuki; Pramod, Poudel; Miyamoto, Hisashi; Sakai, Kenji.

In: Bioresource Technology, Vol. 146, 01.01.2013, p. 672-681.

Research output: Contribution to journalArticle

Tashiro, Yukihiro ; Matsumoto, Hiroko ; Miyamoto, Hirokuni ; Okugawa, Yuki ; Pramod, Poudel ; Miyamoto, Hisashi ; Sakai, Kenji. / A novel production process for optically pure l-lactic acid from kitchen refuse using a bacterial consortium at high temperatures. In: Bioresource Technology. 2013 ; Vol. 146. pp. 672-681.
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