A novel protein with RNA-binding motifs interacts with ataxin-2

Hiroki Shibata, Duong P. Huynh, Stefan M. Pulst

Research output: Contribution to journalArticle

174 Citations (Scopus)

Abstract

Spinocerebellar ataxia type 2 (SCA2) is caused by expansion of a polyglutamine tract in ataxin-2, a protein of unknown function. Using the yeast two-hybrid system, we identified a novel protein, A2BP1 (ataxin-2 binding protein 1) which binds to the C-terminus of ataxin-2. Northern blot analysis showed that A2BP1 was predominantly expressed in muscle and brain. By immunocfluorescent staining, A2BP1 and ataxin-2 were both localized to the trans-Golgi network. Immunocytochemistry showed that A2BP1 was expressed in the cytoplasm of Purkinje cells and dentate neurons in a pattern similar to that seen for ataxin-2 labeling. Western blot analysis of subcellular fractions indicated enrichment of A2BP1 in the same fractions as ataxin-2. Sequence analysis of the A2BP1 cDNA revealed an RNP motif that is highly conserved among RNA-binding proteins. A2BP1 had striking homology with a human cDNA clone, P83A20, of unknown function and at least two copies of A2BP1 homologs are found in the Caenorhabditis elegans genome database. A2BP1 and related proteins appear to form a novel gene family sharing RNA-binding motifs.

Original languageEnglish
Pages (from-to)1303-1313
Number of pages11
JournalHuman Molecular Genetics
Volume9
Issue number9
Publication statusPublished - May 22 2000
Externally publishedYes

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Carrier Proteins
Proteins
Ataxin-2
RNA-Binding Motifs
Complementary DNA
trans-Golgi Network
Spinocerebellar Ataxias
Two-Hybrid System Techniques
RNA-Binding Proteins
Subcellular Fractions
Purkinje Cells
Caenorhabditis elegans
Northern Blotting
Sequence Analysis
Cytoplasm
Clone Cells
Western Blotting
Immunohistochemistry
Genome
Databases

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

A novel protein with RNA-binding motifs interacts with ataxin-2. / Shibata, Hiroki; Huynh, Duong P.; Pulst, Stefan M.

In: Human Molecular Genetics, Vol. 9, No. 9, 22.05.2000, p. 1303-1313.

Research output: Contribution to journalArticle

Shibata, H, Huynh, DP & Pulst, SM 2000, 'A novel protein with RNA-binding motifs interacts with ataxin-2', Human Molecular Genetics, vol. 9, no. 9, pp. 1303-1313.
Shibata, Hiroki ; Huynh, Duong P. ; Pulst, Stefan M. / A novel protein with RNA-binding motifs interacts with ataxin-2. In: Human Molecular Genetics. 2000 ; Vol. 9, No. 9. pp. 1303-1313.
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