A novel small regulatory RNA enhances cell motility in enterohemorrhagic Escherichia coli

Naoki Sudo, Akiko Soma, Akira Muto, Sunao Iyoda, Mayumi Suh, Nanako Kurihara, Hiroyuki Abe, Toru Tobe, Yoshitoshi Ogura, Tetsuya Hayashi, Ken Kurokawa, Makoto Ohnishi, Yasuhiko Sekine

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Abstract

Small regulatory RNAs (sRNAs) are conserved among a wide range of bacteria. They modulate the translational efficiency of target mRNAs through base-pairing with the help of RNA chaperone Hfq. The present study identified a novel sRNA, Esr41 (enterohemorrhagic Escherichia coli O157 small RNA #41), from an intergenic region of an enterohemorrhagic E. coli (EHEC) O157:H7 Sakai-specific sequence that is not present in the nonpathogenic E. coli K-12. Esr41 was detected as an RNA molecule approximately 70 nucleotides long with a 3′ GC-rich palindrome sequence followed by a long poly(U), which is a characteristic of rho-independent terminators and is also a structural feature required for the action of Hfq. EHEC O157 harboring a multicopy plasmid carrying the esr41 gene increased cell motility and the expression of fliC, a gene encoding a major flagellar component. These results indicate that Esr41 stimulates fliC expression in EHEC O157. Furthermore, the increase in cell motility induced by Esr41 was also observed in the E. coli K-12, suggesting that target genes controlled by Esr41 are present in both EHEC O157 and K-12.

Original languageEnglish
Pages (from-to)44-50
Number of pages7
JournalJournal of General and Applied Microbiology
Volume60
Issue number1
DOIs
Publication statusPublished - 2014
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology

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