A Novel Subcutaneous Site of Islet Transplantation Superior to the Liver

Yohichi Yasunami, Yuki Nakafusa, Naoyoshi Nitta, Masafumi Nakamura, Masafumi Goto, Junko Ono, Masaru Taniguchi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background Islet transplantation is an attractive treatment for patients with insulin-dependent diabetes mellitus, and currently, the liver is the favored transplantation site. However, an alternative site is desirable because of the low efficiency of hepatic transplantation, requiring 2 to 3 donors for a single recipient, and because the transplanted islets cannot be accessed or retrieved. Methods We developed a novel procedure of islet transplantation to the inguinal subcutaneous white adipose tissue (ISWAT) of mice and described functional and morphological characteristics of transplanted syngeneic islets. Also, it was determined whether islet allograft rejection in the ISWAT can be prevented by immunosuppressive agents. Furthermore, it was examined whether human islets function when grafted in this particular site of immune-deficient mice. Results In this site, transplanted islets are engrafted as clusters and function to reverse streptozotocin-induced diabetes in mice. Importantly, transplanted islets can be visualized by computed tomography and are easily retrievable, and allograft rejection is preventable by blockade of costimulatory signals. Of much importance, the efficiency of islet transplantation in this site is superior to the liver, in which hyperglycemia of diabetic recipient mice is ameliorated after transplantation of 200 syngeneic islets (the islet number yielded from 1 mouse pancreas) to the ISWAT but not to the liver. Furthermore, human islets transplanted in this particular site function to reverse diabetes in immune-deficient mice. Conclusions Thus, the ISWAT is superior to the liver as the site of islet transplantation, which may lead to improved outcome of clinical islet transplantation.

Original languageEnglish
Pages (from-to)945-952
Number of pages8
JournalTransplantation
Volume102
Issue number6
DOIs
Publication statusPublished - Jun 1 2018

Fingerprint

Islets of Langerhans Transplantation
White Adipose Tissue
Groin
Subcutaneous Fat
Liver
Allografts
Isogeneic Transplantation
Experimental Diabetes Mellitus
Immunosuppressive Agents
Type 1 Diabetes Mellitus
Hyperglycemia
Liver Transplantation
Pancreas
Transplantation
Tomography
Tissue Donors

All Science Journal Classification (ASJC) codes

  • Transplantation

Cite this

Yasunami, Y., Nakafusa, Y., Nitta, N., Nakamura, M., Goto, M., Ono, J., & Taniguchi, M. (2018). A Novel Subcutaneous Site of Islet Transplantation Superior to the Liver. Transplantation, 102(6), 945-952. https://doi.org/10.1097/TP.0000000000002162

A Novel Subcutaneous Site of Islet Transplantation Superior to the Liver. / Yasunami, Yohichi; Nakafusa, Yuki; Nitta, Naoyoshi; Nakamura, Masafumi; Goto, Masafumi; Ono, Junko; Taniguchi, Masaru.

In: Transplantation, Vol. 102, No. 6, 01.06.2018, p. 945-952.

Research output: Contribution to journalArticle

Yasunami, Y, Nakafusa, Y, Nitta, N, Nakamura, M, Goto, M, Ono, J & Taniguchi, M 2018, 'A Novel Subcutaneous Site of Islet Transplantation Superior to the Liver', Transplantation, vol. 102, no. 6, pp. 945-952. https://doi.org/10.1097/TP.0000000000002162
Yasunami, Yohichi ; Nakafusa, Yuki ; Nitta, Naoyoshi ; Nakamura, Masafumi ; Goto, Masafumi ; Ono, Junko ; Taniguchi, Masaru. / A Novel Subcutaneous Site of Islet Transplantation Superior to the Liver. In: Transplantation. 2018 ; Vol. 102, No. 6. pp. 945-952.
@article{3d9240b15a5b4337a8bcf4ac98297b27,
title = "A Novel Subcutaneous Site of Islet Transplantation Superior to the Liver",
abstract = "Background Islet transplantation is an attractive treatment for patients with insulin-dependent diabetes mellitus, and currently, the liver is the favored transplantation site. However, an alternative site is desirable because of the low efficiency of hepatic transplantation, requiring 2 to 3 donors for a single recipient, and because the transplanted islets cannot be accessed or retrieved. Methods We developed a novel procedure of islet transplantation to the inguinal subcutaneous white adipose tissue (ISWAT) of mice and described functional and morphological characteristics of transplanted syngeneic islets. Also, it was determined whether islet allograft rejection in the ISWAT can be prevented by immunosuppressive agents. Furthermore, it was examined whether human islets function when grafted in this particular site of immune-deficient mice. Results In this site, transplanted islets are engrafted as clusters and function to reverse streptozotocin-induced diabetes in mice. Importantly, transplanted islets can be visualized by computed tomography and are easily retrievable, and allograft rejection is preventable by blockade of costimulatory signals. Of much importance, the efficiency of islet transplantation in this site is superior to the liver, in which hyperglycemia of diabetic recipient mice is ameliorated after transplantation of 200 syngeneic islets (the islet number yielded from 1 mouse pancreas) to the ISWAT but not to the liver. Furthermore, human islets transplanted in this particular site function to reverse diabetes in immune-deficient mice. Conclusions Thus, the ISWAT is superior to the liver as the site of islet transplantation, which may lead to improved outcome of clinical islet transplantation.",
author = "Yohichi Yasunami and Yuki Nakafusa and Naoyoshi Nitta and Masafumi Nakamura and Masafumi Goto and Junko Ono and Masaru Taniguchi",
year = "2018",
month = "6",
day = "1",
doi = "10.1097/TP.0000000000002162",
language = "English",
volume = "102",
pages = "945--952",
journal = "Transplantation",
issn = "0041-1337",
publisher = "Lippincott Williams and Wilkins",
number = "6",

}

TY - JOUR

T1 - A Novel Subcutaneous Site of Islet Transplantation Superior to the Liver

AU - Yasunami, Yohichi

AU - Nakafusa, Yuki

AU - Nitta, Naoyoshi

AU - Nakamura, Masafumi

AU - Goto, Masafumi

AU - Ono, Junko

AU - Taniguchi, Masaru

PY - 2018/6/1

Y1 - 2018/6/1

N2 - Background Islet transplantation is an attractive treatment for patients with insulin-dependent diabetes mellitus, and currently, the liver is the favored transplantation site. However, an alternative site is desirable because of the low efficiency of hepatic transplantation, requiring 2 to 3 donors for a single recipient, and because the transplanted islets cannot be accessed or retrieved. Methods We developed a novel procedure of islet transplantation to the inguinal subcutaneous white adipose tissue (ISWAT) of mice and described functional and morphological characteristics of transplanted syngeneic islets. Also, it was determined whether islet allograft rejection in the ISWAT can be prevented by immunosuppressive agents. Furthermore, it was examined whether human islets function when grafted in this particular site of immune-deficient mice. Results In this site, transplanted islets are engrafted as clusters and function to reverse streptozotocin-induced diabetes in mice. Importantly, transplanted islets can be visualized by computed tomography and are easily retrievable, and allograft rejection is preventable by blockade of costimulatory signals. Of much importance, the efficiency of islet transplantation in this site is superior to the liver, in which hyperglycemia of diabetic recipient mice is ameliorated after transplantation of 200 syngeneic islets (the islet number yielded from 1 mouse pancreas) to the ISWAT but not to the liver. Furthermore, human islets transplanted in this particular site function to reverse diabetes in immune-deficient mice. Conclusions Thus, the ISWAT is superior to the liver as the site of islet transplantation, which may lead to improved outcome of clinical islet transplantation.

AB - Background Islet transplantation is an attractive treatment for patients with insulin-dependent diabetes mellitus, and currently, the liver is the favored transplantation site. However, an alternative site is desirable because of the low efficiency of hepatic transplantation, requiring 2 to 3 donors for a single recipient, and because the transplanted islets cannot be accessed or retrieved. Methods We developed a novel procedure of islet transplantation to the inguinal subcutaneous white adipose tissue (ISWAT) of mice and described functional and morphological characteristics of transplanted syngeneic islets. Also, it was determined whether islet allograft rejection in the ISWAT can be prevented by immunosuppressive agents. Furthermore, it was examined whether human islets function when grafted in this particular site of immune-deficient mice. Results In this site, transplanted islets are engrafted as clusters and function to reverse streptozotocin-induced diabetes in mice. Importantly, transplanted islets can be visualized by computed tomography and are easily retrievable, and allograft rejection is preventable by blockade of costimulatory signals. Of much importance, the efficiency of islet transplantation in this site is superior to the liver, in which hyperglycemia of diabetic recipient mice is ameliorated after transplantation of 200 syngeneic islets (the islet number yielded from 1 mouse pancreas) to the ISWAT but not to the liver. Furthermore, human islets transplanted in this particular site function to reverse diabetes in immune-deficient mice. Conclusions Thus, the ISWAT is superior to the liver as the site of islet transplantation, which may lead to improved outcome of clinical islet transplantation.

UR - http://www.scopus.com/inward/record.url?scp=85047990739&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047990739&partnerID=8YFLogxK

U2 - 10.1097/TP.0000000000002162

DO - 10.1097/TP.0000000000002162

M3 - Article

C2 - 29521877

AN - SCOPUS:85047990739

VL - 102

SP - 945

EP - 952

JO - Transplantation

JF - Transplantation

SN - 0041-1337

IS - 6

ER -