Abstract
Schizophyllan (SPG) is a kind of beta-1,3-glucan which has glucose side chain through a beta-1,6-glycosil bond (Fig. 1). It was found that SPG could form complex with single stranded polynucleotides. SPG complexes with single stranded RNAs that have similar helix parameter to triple stranded SPG thorough hydrogen bonding and hydrophobic interaction. We prepared single stranded SPG appended gel supports. It worked as affinity column for polynucleotides. Poly(C) and poly(A) was retained in the SPG column at low temperature, though poly(G) passed by SPG column without interaction. RNAs trapped in the SPG appended column were released by changing elution buffer under mild condition. This SPG column could use to separation of mRNA which has poly(A) tail. The results obtained led to the conclusion that SPG which has neither cation charge or nucleic base can play as RNA separation material.
| Original language | English |
|---|---|
| Pages (from-to) | 283-284 |
| Number of pages | 2 |
| Journal | Nucleic acids research. Supplement (2001) |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 2001 |
| Externally published | Yes |
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A novel technique to specifically separate RNAs by Schizophyllan. / Kimura, T.; Koumoto, K.; Mizu, M.; Kobayashi, R.; Sakurai, K.; Shinkai, S.
In: Nucleic acids research. Supplement (2001), No. 1, 01.01.2001, p. 283-284.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A novel technique to specifically separate RNAs by Schizophyllan.
AU - Kimura, T.
AU - Koumoto, K.
AU - Mizu, M.
AU - Kobayashi, R.
AU - Sakurai, K.
AU - Shinkai, S.
PY - 2001/1/1
Y1 - 2001/1/1
N2 - Schizophyllan (SPG) is a kind of beta-1,3-glucan which has glucose side chain through a beta-1,6-glycosil bond (Fig. 1). It was found that SPG could form complex with single stranded polynucleotides. SPG complexes with single stranded RNAs that have similar helix parameter to triple stranded SPG thorough hydrogen bonding and hydrophobic interaction. We prepared single stranded SPG appended gel supports. It worked as affinity column for polynucleotides. Poly(C) and poly(A) was retained in the SPG column at low temperature, though poly(G) passed by SPG column without interaction. RNAs trapped in the SPG appended column were released by changing elution buffer under mild condition. This SPG column could use to separation of mRNA which has poly(A) tail. The results obtained led to the conclusion that SPG which has neither cation charge or nucleic base can play as RNA separation material.
AB - Schizophyllan (SPG) is a kind of beta-1,3-glucan which has glucose side chain through a beta-1,6-glycosil bond (Fig. 1). It was found that SPG could form complex with single stranded polynucleotides. SPG complexes with single stranded RNAs that have similar helix parameter to triple stranded SPG thorough hydrogen bonding and hydrophobic interaction. We prepared single stranded SPG appended gel supports. It worked as affinity column for polynucleotides. Poly(C) and poly(A) was retained in the SPG column at low temperature, though poly(G) passed by SPG column without interaction. RNAs trapped in the SPG appended column were released by changing elution buffer under mild condition. This SPG column could use to separation of mRNA which has poly(A) tail. The results obtained led to the conclusion that SPG which has neither cation charge or nucleic base can play as RNA separation material.
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UR - http://www.scopus.com/inward/citedby.url?scp=0043205927&partnerID=8YFLogxK
U2 - 10.1093/nass/1.1.283
DO - 10.1093/nass/1.1.283
M3 - Article
C2 - 12836375
AN - SCOPUS:0043205927
SP - 283
EP - 284
JO - Nucleic acids research. Supplement (2001)
JF - Nucleic acids research. Supplement (2001)
IS - 1
ER -