A novel technique to specifically separate RNAs by Schizophyllan.

T. Kimura, K. Koumoto, M. Mizu, R. Kobayashi, K. Sakurai, S. Shinkai

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Schizophyllan (SPG) is a kind of beta-1,3-glucan which has glucose side chain through a beta-1,6-glycosil bond (Fig. 1). It was found that SPG could form complex with single stranded polynucleotides. SPG complexes with single stranded RNAs that have similar helix parameter to triple stranded SPG thorough hydrogen bonding and hydrophobic interaction. We prepared single stranded SPG appended gel supports. It worked as affinity column for polynucleotides. Poly(C) and poly(A) was retained in the SPG column at low temperature, though poly(G) passed by SPG column without interaction. RNAs trapped in the SPG appended column were released by changing elution buffer under mild condition. This SPG column could use to separation of mRNA which has poly(A) tail. The results obtained led to the conclusion that SPG which has neither cation charge or nucleic base can play as RNA separation material.

Original languageEnglish
Pages (from-to)283-284
Number of pages2
JournalNucleic acids research. Supplement (2001)
Issue number1
DOIs
Publication statusPublished - Jan 1 2001
Externally publishedYes

Fingerprint

Sizofiran
Polynucleotides
RNA
Poly G
Messenger RNA
Poly A
Hydrogen Bonding
Hydrophobic and Hydrophilic Interactions
Cations
Buffers
Gels
Glucose
Temperature

Cite this

Kimura, T., Koumoto, K., Mizu, M., Kobayashi, R., Sakurai, K., & Shinkai, S. (2001). A novel technique to specifically separate RNAs by Schizophyllan. Nucleic acids research. Supplement (2001), (1), 283-284. https://doi.org/10.1093/nass/1.1.283

A novel technique to specifically separate RNAs by Schizophyllan. / Kimura, T.; Koumoto, K.; Mizu, M.; Kobayashi, R.; Sakurai, K.; Shinkai, S.

In: Nucleic acids research. Supplement (2001), No. 1, 01.01.2001, p. 283-284.

Research output: Contribution to journalArticle

Kimura, T, Koumoto, K, Mizu, M, Kobayashi, R, Sakurai, K & Shinkai, S 2001, 'A novel technique to specifically separate RNAs by Schizophyllan.', Nucleic acids research. Supplement (2001), no. 1, pp. 283-284. https://doi.org/10.1093/nass/1.1.283
Kimura, T. ; Koumoto, K. ; Mizu, M. ; Kobayashi, R. ; Sakurai, K. ; Shinkai, S. / A novel technique to specifically separate RNAs by Schizophyllan. In: Nucleic acids research. Supplement (2001). 2001 ; No. 1. pp. 283-284.
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