A novel transfection method for mammalian cells using gas plasma

Yasunari Sakai, Vahid Khajoee, Yasuhiro Ogawa, Koichi Kusuhara, Yoshiki Katayama, Toshiro Hara

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

Introduction of foreign genes into target cells is a crucial step for achievement of gene therapy. We have recently developed a novel transfection system for eukaryotic cells, namely the electric pulse-activated gas plasma generator. To measure the transfection efficiency and mortality by flow-cytometry, we employed enhanced green fluorescent protein and propidium iodide staining, respectively. One day after the 1-3 s plasma exposures with DNA concentration at 0.5 μg/μl, favorable transfection efficiencies (17.8-21.6%) and mortalities (0.65-2.86%) were obtained for HeLa-S3, HT-1080 and MCF-7 cells. The recipient cells became transiently permeable for plasmid DNA during the plasma exposure, suggesting that plasma-mediated transfection may involve similar mechanisms that accounts for electroporation. The relatively low mortality rates are encouraging in our attempt to apply this system to the various cell lines including the primary cell cultures.

Original languageEnglish
Pages (from-to)299-308
Number of pages10
JournalJournal of Biotechnology
Volume121
Issue number3
DOIs
Publication statusPublished - Feb 10 2006

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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