A novel type of immunity protein, NukH, for the lantibiotic nukacin ISK-1 produced by Staphylococcus warneri ISK-1

Yuji Aso, Ken Ichi Okuda, Jun Ichi Nagao, Youhei Kanemasa, Nguyen Thi Bich Phuong, Hanako Koga, Kouki Shioya, Toshihiro Sashihara, Jiro Nakayama, Kenji Sonomoto

Research output: Contribution to journalArticle

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Abstract

Staphylococcus warneri ISK-1 produces a lantibiotic, nukacin ISK-1. The nukacin ISK-1 gene cluster consists of at least six genes, nukA, -M, -T, -F, -E, and -G, and two open reading frames, ORF1 and ORF7 (designated nukH). Sequence comparisons suggested that NukF, -E, -G, and -H contribute to immunity to nukacin ISK-1. We investigated the immunity levels of recombinant Lactococcus lactis expressing nukFEG and nukH against nukacin ISK-1. The co-expression of nukFEG and nukH resulted in a high degree of immunity. The expression of either nukFEG or nukH conferred partial immunity against nukacin ISK-1. These results suggest that NukH contributes cooperatively to self-protection with Nuk-FEG. The nukacin ISK-1 immunity system might function against another lantibiotic, lacticin 481. Western blot analysis showed that NukH expressed in Staphylococcus carnosus was localized in the membrane. Peptide release/bind assays indicated that the recombinant L. lactis expressing nukH interacted with nukacin ISK-1 and lacticin 481 but not with nisin A. These findings suggest that NukH contributes cooperatively to host immunity as a novel type of lantibiotic-binding immunity protein with NukFEG.

Original languageEnglish
Pages (from-to)1403-1410
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume69
Issue number7
DOIs
Publication statusPublished - Sep 1 2005

Fingerprint

Bacteriocins
Staphylococcus
Immunity
Proteins
Lactococcus lactis
Genes
Multigene Family
nukacin ISK-1
Open Reading Frames
Assays
Carrier Proteins
Western Blotting
Membranes
Peptides

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Aso, Y., Okuda, K. I., Nagao, J. I., Kanemasa, Y., Thi Bich Phuong, N., Koga, H., ... Sonomoto, K. (2005). A novel type of immunity protein, NukH, for the lantibiotic nukacin ISK-1 produced by Staphylococcus warneri ISK-1. Bioscience, Biotechnology and Biochemistry, 69(7), 1403-1410. https://doi.org/10.1271/bbb.69.1403

A novel type of immunity protein, NukH, for the lantibiotic nukacin ISK-1 produced by Staphylococcus warneri ISK-1. / Aso, Yuji; Okuda, Ken Ichi; Nagao, Jun Ichi; Kanemasa, Youhei; Thi Bich Phuong, Nguyen; Koga, Hanako; Shioya, Kouki; Sashihara, Toshihiro; Nakayama, Jiro; Sonomoto, Kenji.

In: Bioscience, Biotechnology and Biochemistry, Vol. 69, No. 7, 01.09.2005, p. 1403-1410.

Research output: Contribution to journalArticle

Aso, Y, Okuda, KI, Nagao, JI, Kanemasa, Y, Thi Bich Phuong, N, Koga, H, Shioya, K, Sashihara, T, Nakayama, J & Sonomoto, K 2005, 'A novel type of immunity protein, NukH, for the lantibiotic nukacin ISK-1 produced by Staphylococcus warneri ISK-1', Bioscience, Biotechnology and Biochemistry, vol. 69, no. 7, pp. 1403-1410. https://doi.org/10.1271/bbb.69.1403
Aso, Yuji ; Okuda, Ken Ichi ; Nagao, Jun Ichi ; Kanemasa, Youhei ; Thi Bich Phuong, Nguyen ; Koga, Hanako ; Shioya, Kouki ; Sashihara, Toshihiro ; Nakayama, Jiro ; Sonomoto, Kenji. / A novel type of immunity protein, NukH, for the lantibiotic nukacin ISK-1 produced by Staphylococcus warneri ISK-1. In: Bioscience, Biotechnology and Biochemistry. 2005 ; Vol. 69, No. 7. pp. 1403-1410.
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