A nuclear factor which interacts with an at-cluster in the first intron of rat α2-macroglobulin gene

Takashi Ito, Yoshiyuki Sakaki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We have identified a nuclear protein which binds specifically to the first intron of rat α2-macroglobulin gene. The protein became insoluble at low salt concentration retaining the binding specificity. Its molecular weight was estimated to be 22 kilodalton by a protein blotting procedure. The binding site of the protein determined by DNase I footprinting was an AT-strech which shared 80% homology with the cleavage consensus of Drosophila topoisomerase II.

Original languageEnglish
Pages (from-to)824-830
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume147
Issue number2
DOIs
Publication statusPublished - Sep 15 1987

Fingerprint

Macroglobulins
Introns
Rats
Genes
Type II DNA Topoisomerase
Deoxyribonuclease I
Nuclear Proteins
Drosophila
Carrier Proteins
Proteins
Salts
Molecular Weight
Binding Sites
Molecular weight

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

A nuclear factor which interacts with an at-cluster in the first intron of rat α2-macroglobulin gene. / Ito, Takashi; Sakaki, Yoshiyuki.

In: Biochemical and Biophysical Research Communications, Vol. 147, No. 2, 15.09.1987, p. 824-830.

Research output: Contribution to journalArticle

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