A one-step immunochromatographic assay for detecting ginsenosides Rb1 and Rg1

Waraporn Putalun; Noriko Fukuda; Hiroyuki Tanaka; Yukihiro Shoyama

doi:10.1007/s00216-003-2408-x

A one-step immunochromatographic assay for detecting ginsenosides Rb1 and Rg1

Waraporn Putalun, Noriko Fukuda, Hiroyuki Tanaka, Yukihiro Shoyama

Pharmaceutical Health Care and Sciences

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

An immunochromatographic strip test has been developed for detecting ginsenosides Rb1 (G-Rb1) and Rg1 (G-Rg1). This qualitative assay system is useful as a rapid screening method for detecting G-Rb1 and G-Rg1 in plants and plant preparations. Our assay is a competitive immunoassay that uses anti-G-Rb1 and anti-G-Rg1 monoclonal antibodies (MAbs) and a detection reagent that contains colloidal gold particles coated with anti-G-Rb1 and anti-G-Rg1 MAbs. Detection limits are 2 μg mL^-1 for both G-Rb1 and G-Rg1.

Original language	English
Pages (from-to)	1338-1341
Number of pages	4
Journal	Analytical and Bioanalytical Chemistry
Volume	378
Issue number	5
DOIs	https://doi.org/10.1007/s00216-003-2408-x
Publication status	Published - Mar 2004

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry

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abstract = "An immunochromatographic strip test has been developed for detecting ginsenosides Rb1 (G-Rb1) and Rg1 (G-Rg1). This qualitative assay system is useful as a rapid screening method for detecting G-Rb1 and G-Rg1 in plants and plant preparations. Our assay is a competitive immunoassay that uses anti-G-Rb1 and anti-G-Rg1 monoclonal antibodies (MAbs) and a detection reagent that contains colloidal gold particles coated with anti-G-Rb1 and anti-G-Rg1 MAbs. Detection limits are 2 μg mL-1 for both G-Rb1 and G-Rg1.",

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A one-step immunochromatographic assay for detecting ginsenosides Rb1 and Rg1

Abstract

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