A one-step immunochromatographic assay for detecting ginsenosides Rb1 and Rg1

Waraporn Putalun, Noriko Fukuda, Hiroyuki Tanaka, Yukihiro Shoyama

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Abstract

An immunochromatographic strip test has been developed for detecting ginsenosides Rb1 (G-Rb1) and Rg1 (G-Rg1). This qualitative assay system is useful as a rapid screening method for detecting G-Rb1 and G-Rg1 in plants and plant preparations. Our assay is a competitive immunoassay that uses anti-G-Rb1 and anti-G-Rg1 monoclonal antibodies (MAbs) and a detection reagent that contains colloidal gold particles coated with anti-G-Rb1 and anti-G-Rg1 MAbs. Detection limits are 2 μg mL-1 for both G-Rb1 and G-Rg1.

Original languageEnglish
Pages (from-to)1338-1341
Number of pages4
JournalAnalytical and Bioanalytical Chemistry
Volume378
Issue number5
DOIs
Publication statusPublished - Mar 1 2004

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

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