TY - JOUR
T1 - A palm-sized surface plasmon resonance sensor with microchip flow cell
AU - Nakajima, Hizuru
AU - Harada, Yuuki
AU - Asano, Yasukazu
AU - Nakagama, Tatsuro
AU - Uchiyama, Katsumi
AU - Imato, Toshihiko
AU - Soh, Nobuaki
AU - Hemmi, Akihide
N1 - Funding Information:
This research was partly supported by a Grant-in-Aid for Scientific Research (No. 16350047) from the Japan Society for the Promotion of Science (JSPS).
PY - 2006/9/15
Y1 - 2006/9/15
N2 - A small-sized surface plasmon resonance (SPR) sensor with a microchip flow cell has been developed for the purpose of enhancing the sensitivity of the SPR detector for low molecular weight compounds. This portable differential SPR detector consisted of an LED, two cylindrical lenses, a round prism, a divided mirror, a CCD, electronics, and a polydimethylsiloxane/gold microchip with two flow paths (10 mm long, 1 mm wide, 20-100 μm deep). 3-Mercaptopropyltrimethoxysilane was used for sealing the microchip. The performance of the on-site orientated SPR detector was estimated using sucrose and IgA. A drastic change in the SPR intensity appeared. The depth of the flow cell was in inverse proportion to the SPR intensity. Compared to a conventional flow cell having the size of 10 mm (L) × 1 mm (W) × 1 mm (D), its sensitivity to 10% sucrose and 0.9 nM IgA increased about 11 and 39 times, respectively. This phenomenon seemed to be due to the increase in the substance on the SPR sensor based on its size effect. These results showed that the application of the microchip sensor for SPR measurement has the possibility for improvement of the SPR intensity for low molecular substances.
AB - A small-sized surface plasmon resonance (SPR) sensor with a microchip flow cell has been developed for the purpose of enhancing the sensitivity of the SPR detector for low molecular weight compounds. This portable differential SPR detector consisted of an LED, two cylindrical lenses, a round prism, a divided mirror, a CCD, electronics, and a polydimethylsiloxane/gold microchip with two flow paths (10 mm long, 1 mm wide, 20-100 μm deep). 3-Mercaptopropyltrimethoxysilane was used for sealing the microchip. The performance of the on-site orientated SPR detector was estimated using sucrose and IgA. A drastic change in the SPR intensity appeared. The depth of the flow cell was in inverse proportion to the SPR intensity. Compared to a conventional flow cell having the size of 10 mm (L) × 1 mm (W) × 1 mm (D), its sensitivity to 10% sucrose and 0.9 nM IgA increased about 11 and 39 times, respectively. This phenomenon seemed to be due to the increase in the substance on the SPR sensor based on its size effect. These results showed that the application of the microchip sensor for SPR measurement has the possibility for improvement of the SPR intensity for low molecular substances.
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U2 - 10.1016/j.talanta.2006.02.068
DO - 10.1016/j.talanta.2006.02.068
M3 - Article
C2 - 18970786
AN - SCOPUS:33747111318
SN - 0039-9140
VL - 70
SP - 419
EP - 425
JO - Talanta
JF - Talanta
IS - 2
ER -