A panel of specific monoclonal antibodies directed against various phosphorylated histones H3

Tomohiko Yoshimi, Yasuyuki Ohkawa, Masayuki Azuma, Taro Tachibana

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    1 Citation (Scopus)

    Abstract

    Modification of histone plays a critical role in the epigenetic regulation of gene expression. However, unlike the widely studied roles of histone methylation or acetylation of histone H3, relatively little is known about the molecular mechanisms involved in translating histone phosphorylation into a specific outcome. The present study reports on the development of antibodies (MAbs) directed against phosphorylated histone H3 (S10, T11, S28, S31, and T32), produced by the hybridization of mouse myeloma cells with lymph node cells from an immunized rat or mouse. The MAbs produced specifically recognize different sites of phosphorylation on histone H3. All of these MAbs are suitable for immunoblotting and immunofluorescence analysis. We believe that these antibodies should significantly facilitate our efforts to investigate epigenetic regulation.

    Original languageEnglish
    Pages (from-to)119-124
    Number of pages6
    JournalMonoclonal Antibodies in Immunodiagnosis and Immunotherapy
    Volume32
    Issue number2
    DOIs
    Publication statusPublished - Apr 1 2013

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

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