A peptide microarray for the detection of protein kinase activity in cell lysate

Syuhei Shigaki, Takayuki Yamaji, Xiaoming Han, Go Yamanouchi, Tatsuhiko Sonoda, Osamu Okitsu, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

DNA microarray enables the analysis of DNA or mRNA expression levels, but it has not been possible to completely understand life using obtained information. Consequently, protein or peptide arrays have attracted much interest. Since the development of a practical protein microarray is still far away in light of handling difficulties, the peptide microarray is a promising tool for analyzing protein functions. We have developed a peptide microarray to detect protein kinase activity in cell lysate. All substrate peptides for kinases were immobilized chemoselectively on amino-coated glass slides. After phosphorylation of the immobilized peptides, phosphorylation was detected by fluorescence imaging. We detected the protein kinase activities, including that in cell lysate, in response to drug stimulation. Therefore, this peptide microarray would be useful for a high-throughput kinase assay of intracellular signals and would be applicable to drug screening. 2007

Original languageEnglish
Pages (from-to)271-275
Number of pages5
JournalAnalytical Sciences
Volume23
Issue number3
DOIs
Publication statusPublished - Mar 1 2007

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Microarrays
Protein Kinases
Peptides
Phosphorylation
Phosphotransferases
Proteins
DNA
Pharmaceutical Preparations
Assays
Screening
Fluorescence
Throughput
Imaging techniques
Glass
Messenger RNA
Substrates

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

A peptide microarray for the detection of protein kinase activity in cell lysate. / Shigaki, Syuhei; Yamaji, Takayuki; Han, Xiaoming; Yamanouchi, Go; Sonoda, Tatsuhiko; Okitsu, Osamu; Mori, Takeshi; Niidome, Takuro; Katayama, Yoshiki.

In: Analytical Sciences, Vol. 23, No. 3, 01.03.2007, p. 271-275.

Research output: Contribution to journalArticle

Shigaki, S, Yamaji, T, Han, X, Yamanouchi, G, Sonoda, T, Okitsu, O, Mori, T, Niidome, T & Katayama, Y 2007, 'A peptide microarray for the detection of protein kinase activity in cell lysate', Analytical Sciences, vol. 23, no. 3, pp. 271-275. https://doi.org/10.2116/analsci.23.271
Shigaki S, Yamaji T, Han X, Yamanouchi G, Sonoda T, Okitsu O et al. A peptide microarray for the detection of protein kinase activity in cell lysate. Analytical Sciences. 2007 Mar 1;23(3):271-275. https://doi.org/10.2116/analsci.23.271
Shigaki, Syuhei ; Yamaji, Takayuki ; Han, Xiaoming ; Yamanouchi, Go ; Sonoda, Tatsuhiko ; Okitsu, Osamu ; Mori, Takeshi ; Niidome, Takuro ; Katayama, Yoshiki. / A peptide microarray for the detection of protein kinase activity in cell lysate. In: Analytical Sciences. 2007 ; Vol. 23, No. 3. pp. 271-275.
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