A peptide microarray for the detection of protein kinase activity in cell lysate

Syuhei Shigaki, Takayuki Yamaji, Xiaoming Han, Go Yamanouchi, Tatsuhiko Sonoda, Osamu Okitsu, Takeshi Mori, Takuro Niidome, Yoshiki Katayama

    Research output: Contribution to journalArticlepeer-review

    33 Citations (Scopus)

    Abstract

    DNA microarray enables the analysis of DNA or mRNA expression levels, but it has not been possible to completely understand life using obtained information. Consequently, protein or peptide arrays have attracted much interest. Since the development of a practical protein microarray is still far away in light of handling difficulties, the peptide microarray is a promising tool for analyzing protein functions. We have developed a peptide microarray to detect protein kinase activity in cell lysate. All substrate peptides for kinases were immobilized chemoselectively on amino-coated glass slides. After phosphorylation of the immobilized peptides, phosphorylation was detected by fluorescence imaging. We detected the protein kinase activities, including that in cell lysate, in response to drug stimulation. Therefore, this peptide microarray would be useful for a high-throughput kinase assay of intracellular signals and would be applicable to drug screening. 2007

    Original languageEnglish
    Pages (from-to)271-275
    Number of pages5
    Journalanalytical sciences
    Volume23
    Issue number3
    DOIs
    Publication statusPublished - Mar 2007

    All Science Journal Classification (ASJC) codes

    • Analytical Chemistry

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