A photoregulated ATP generation system for in vitro motility assay

Arif Md Rashedul Kabir, Masaki Ito, Kyohei Uenishi, Shizuka Anan, Akihiko Konagaya, Kazuki Sada, Miwa Sugiura, Akira Kakugo

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Abstract

We have demonstrated in vitro motility assay of microtubules on a kinesin-coated substrate by using adenosine triphosphate (ATP) generated from microsphere gels containing thylakoid membranes. Upon photoirradiation, the gels generated ATP, which was utilized for performing gliding motions of microtubules. This work may facilitate the development of a localized ATP generation system in the in vitro motility assay, which consequently would widen the applications of biomolecular motors in nanotechnology.

Original languageEnglish
Pages (from-to)178-180
Number of pages3
JournalChemistry Letters
Volume46
Issue number2
DOIs
Publication statusPublished - Jan 1 2017
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Kabir, A. M. R., Ito, M., Uenishi, K., Anan, S., Konagaya, A., Sada, K., ... Kakugo, A. (2017). A photoregulated ATP generation system for in vitro motility assay. Chemistry Letters, 46(2), 178-180. https://doi.org/10.1246/cl.160903