A pivot-hinge-style DNA immobilization method with adaptable surface concentration based on oligodeoxynucleotide-phosphorothioate chemisorption on gold surfaces

Hisao Yoshinaga, Koji Nakano, Nobuaki Soh, Ryoichi Ishimatsu, Toshihiko Imato

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The chemisorption of oligodeoxynucleotide phosphorothioate (s-oligo) is reported. A series of s-oligo DNAs was designed for use as capture probe DNA molecules. The s-oligo DNAs consist of the K-ras gene (5'-GGA GCT GGT GGC-3') and a dodecamer deoxyriboadenosine, both of which lie on either side of an s-oligo DNA sequence. By primarily focusing on the capture probe DNA having five-successive s-oligo sequences, e37, the immobilization chemistry of e37 was examined; atomic force microscopy achieved the direct visualization of individual molecules on Au(111) substrates, while a series of surface analyses, including IR, ellipsometry, and microgravimetry, showed that the s-oligo functional groups played a pivotal role in the surface-adlayer through the gold-thiol interaction. Interestingly, the amount of immobilization showed a definite relationship with the number of s-oligo linkages introduced, which should be important to regulate the concentration of the capture probe DNA molecules on the surface. Some preliminary studies using ferrocene-modified complementary sequences indicated that electrochemical labeling and readouts were possible.

Original languageEnglish
Pages (from-to)1059-1064
Number of pages6
Journalanalytical sciences
Volume28
Issue number11
DOIs
Publication statusPublished - Dec 20 2012

Fingerprint

Oligodeoxyribonucleotides
DNA Probes
Hinges
Chemisorption
Gold
Molecules
DNA
DNA sequences
Ellipsometry
Sulfhydryl Compounds
Labeling
Functional groups
Atomic force microscopy
Visualization
Genes
Substrates

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

A pivot-hinge-style DNA immobilization method with adaptable surface concentration based on oligodeoxynucleotide-phosphorothioate chemisorption on gold surfaces. / Yoshinaga, Hisao; Nakano, Koji; Soh, Nobuaki; Ishimatsu, Ryoichi; Imato, Toshihiko.

In: analytical sciences, Vol. 28, No. 11, 20.12.2012, p. 1059-1064.

Research output: Contribution to journalArticle

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