Abstract
New polymer-peptide conjugates, NIPAM-PEP and NIPAM-PEPEP, were designed and synthesized. These graft-type polymers contained a substrate peptide of protein kinase A (PKA), which forms one of the most important intracellular signals in cellular signal transduction. The NIPAM-PEP containing the N-isopropylacrylamide unit and the substrate peptide unit raised its lower critical solution temperature (LCST) from 36.7 to 40 °C in response to phosphorylation by activated PKA. The NIPAM-PEPEP containing another poly(ethylene glycol) unit formed a polymer micelle-type particle above LCST. This particle disintegrated in response to phosphorylation by activated PKA. Dansylaniline, which was encapsulated in the hydrophobic core of the particle, tended to be released with the particle disintegration occurring in response to the PKA signal. NIPAM-PEPEP, which is the first example of a polymer-peptide conjugate responding to an intracellular signal, offers the possibility of a novel, intelligent drug capsule communicating with cells to release its pharmacological activity.
| Original language | English |
|---|---|
| Pages (from-to) | 8569-8573 |
| Number of pages | 5 |
| Journal | Macromolecules |
| Volume | 34 |
| Issue number | 24 |
| DOIs | |
| Publication status | Published - Nov 20 2001 |
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All Science Journal Classification (ASJC) codes
- Organic Chemistry
- Polymers and Plastics
- Inorganic Chemistry
- Materials Chemistry
Cite this
A polymer micelle responding to the protein kinase a signal. / Katayama, Y.; Sonoda, T.; Maeda, M.
In: Macromolecules, Vol. 34, No. 24, 20.11.2001, p. 8569-8573.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A polymer micelle responding to the protein kinase a signal
AU - Katayama, Y.
AU - Sonoda, T.
AU - Maeda, M.
PY - 2001/11/20
Y1 - 2001/11/20
N2 - New polymer-peptide conjugates, NIPAM-PEP and NIPAM-PEPEP, were designed and synthesized. These graft-type polymers contained a substrate peptide of protein kinase A (PKA), which forms one of the most important intracellular signals in cellular signal transduction. The NIPAM-PEP containing the N-isopropylacrylamide unit and the substrate peptide unit raised its lower critical solution temperature (LCST) from 36.7 to 40 °C in response to phosphorylation by activated PKA. The NIPAM-PEPEP containing another poly(ethylene glycol) unit formed a polymer micelle-type particle above LCST. This particle disintegrated in response to phosphorylation by activated PKA. Dansylaniline, which was encapsulated in the hydrophobic core of the particle, tended to be released with the particle disintegration occurring in response to the PKA signal. NIPAM-PEPEP, which is the first example of a polymer-peptide conjugate responding to an intracellular signal, offers the possibility of a novel, intelligent drug capsule communicating with cells to release its pharmacological activity.
AB - New polymer-peptide conjugates, NIPAM-PEP and NIPAM-PEPEP, were designed and synthesized. These graft-type polymers contained a substrate peptide of protein kinase A (PKA), which forms one of the most important intracellular signals in cellular signal transduction. The NIPAM-PEP containing the N-isopropylacrylamide unit and the substrate peptide unit raised its lower critical solution temperature (LCST) from 36.7 to 40 °C in response to phosphorylation by activated PKA. The NIPAM-PEPEP containing another poly(ethylene glycol) unit formed a polymer micelle-type particle above LCST. This particle disintegrated in response to phosphorylation by activated PKA. Dansylaniline, which was encapsulated in the hydrophobic core of the particle, tended to be released with the particle disintegration occurring in response to the PKA signal. NIPAM-PEPEP, which is the first example of a polymer-peptide conjugate responding to an intracellular signal, offers the possibility of a novel, intelligent drug capsule communicating with cells to release its pharmacological activity.
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U2 - 10.1021/ma010966a
DO - 10.1021/ma010966a
M3 - Article
AN - SCOPUS:0035923456
VL - 34
SP - 8569
EP - 8573
JO - Macromolecules
JF - Macromolecules
SN - 0024-9297
IS - 24
ER -