A polysaccharide carrier for immunostimulatory CpG DNAs to enhance cytokine secretion

Masami Mizu, Kazuya Koumoto, Takahisa Anada, Takahiro Matsumoto, Munenori Numata, Seiji Shinkai, Takeshi Nagasaki, Kazuo Sakurai

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

A β-(1 → 3)-D-glucan schizophyllan (SPG) forms a stoichiometric complex with some polynucleotides. This communication describes our attempt to apply the SPG complex to deliver CpG DNA to endosomes to enhance cytokine secretion. To increase cellular uptake, we introduced spermine, arginine-glycine-aspartic acid tripeptide, octaarginine, or cholesterol to the SPG side chain. The chemically modified SPG showed essentially no cytotoxicity. When CpG DNA complex made therefrom was exposed to macrophages, dramatic enhancement in the cytokine secretion was observed. It increased 5-10 times from the naked dose and 100 times from the background. This performance promises that SPG can be an excellent carrier for CpG DNA.

Original languageEnglish
Pages (from-to)8372-8373
Number of pages2
JournalJournal of the American Chemical Society
Volume126
Issue number27
DOIs
Publication statusPublished - Jul 14 2004

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Polysaccharides
DNA
Cytokines
Sizofiran
Polynucleotides
Arginine
Spermine
Macrophages
Endosomes
Cholesterol
Cytotoxicity
Aspartic Acid
Glycine
Amino acids
Acids
Communication

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

A polysaccharide carrier for immunostimulatory CpG DNAs to enhance cytokine secretion. / Mizu, Masami; Koumoto, Kazuya; Anada, Takahisa; Matsumoto, Takahiro; Numata, Munenori; Shinkai, Seiji; Nagasaki, Takeshi; Sakurai, Kazuo.

In: Journal of the American Chemical Society, Vol. 126, No. 27, 14.07.2004, p. 8372-8373.

Research output: Contribution to journalArticle

Mizu, Masami ; Koumoto, Kazuya ; Anada, Takahisa ; Matsumoto, Takahiro ; Numata, Munenori ; Shinkai, Seiji ; Nagasaki, Takeshi ; Sakurai, Kazuo. / A polysaccharide carrier for immunostimulatory CpG DNAs to enhance cytokine secretion. In: Journal of the American Chemical Society. 2004 ; Vol. 126, No. 27. pp. 8372-8373.
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