A polysaccharide carrier for immunostimulatory CpG DNAs to enhance cytokine secretion

Masami Mizu; Kazuya Koumoto; Takahisa Anada; Takahiro Matsumoto; Munenori Numata; Seiji Shinkai; Takeshi Nagasaki; Kazuo Sakurai

doi:10.1021/ja031978+

A polysaccharide carrier for immunostimulatory CpG DNAs to enhance cytokine secretion

Masami Mizu, Kazuya Koumoto, Takahisa Anada, Takahiro Matsumoto, Munenori Numata, Seiji Shinkai, Takeshi Nagasaki, Kazuo Sakurai

Research output: Contribution to journal › Article

44 Citations (Scopus)

Abstract

A β-(1 → 3)-D-glucan schizophyllan (SPG) forms a stoichiometric complex with some polynucleotides. This communication describes our attempt to apply the SPG complex to deliver CpG DNA to endosomes to enhance cytokine secretion. To increase cellular uptake, we introduced spermine, arginine-glycine-aspartic acid tripeptide, octaarginine, or cholesterol to the SPG side chain. The chemically modified SPG showed essentially no cytotoxicity. When CpG DNA complex made therefrom was exposed to macrophages, dramatic enhancement in the cytokine secretion was observed. It increased 5-10 times from the naked dose and 100 times from the background. This performance promises that SPG can be an excellent carrier for CpG DNA.

Original language	English
Pages (from-to)	8372-8373
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	126
Issue number	27
DOIs	https://doi.org/10.1021/ja031978+
Publication status	Published - Jul 14 2004

All Science Journal Classification (ASJC) codes

Chemistry(all)

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10.1021/ja031978+

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Mizu, M., Koumoto, K., Anada, T., Matsumoto, T., Numata, M., Shinkai, S., Nagasaki, T., & Sakurai, K. (2004). A polysaccharide carrier for immunostimulatory CpG DNAs to enhance cytokine secretion. Journal of the American Chemical Society, 126(27), 8372-8373. https://doi.org/10.1021/ja031978+

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abstract = "A β-(1 → 3)-D-glucan schizophyllan (SPG) forms a stoichiometric complex with some polynucleotides. This communication describes our attempt to apply the SPG complex to deliver CpG DNA to endosomes to enhance cytokine secretion. To increase cellular uptake, we introduced spermine, arginine-glycine-aspartic acid tripeptide, octaarginine, or cholesterol to the SPG side chain. The chemically modified SPG showed essentially no cytotoxicity. When CpG DNA complex made therefrom was exposed to macrophages, dramatic enhancement in the cytokine secretion was observed. It increased 5-10 times from the naked dose and 100 times from the background. This performance promises that SPG can be an excellent carrier for CpG DNA.",

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AU - Shinkai, Seiji

AU - Nagasaki, Takeshi

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