Abstract
A β-(1 → 3)-D-glucan schizophyllan (SPG) forms a stoichiometric complex with some polynucleotides. This communication describes our attempt to apply the SPG complex to deliver CpG DNA to endosomes to enhance cytokine secretion. To increase cellular uptake, we introduced spermine, arginine-glycine-aspartic acid tripeptide, octaarginine, or cholesterol to the SPG side chain. The chemically modified SPG showed essentially no cytotoxicity. When CpG DNA complex made therefrom was exposed to macrophages, dramatic enhancement in the cytokine secretion was observed. It increased 5-10 times from the naked dose and 100 times from the background. This performance promises that SPG can be an excellent carrier for CpG DNA.
| Original language | English |
|---|---|
| Pages (from-to) | 8372-8373 |
| Number of pages | 2 |
| Journal | Journal of the American Chemical Society |
| Volume | 126 |
| Issue number | 27 |
| DOIs | |
| Publication status | Published - Jul 14 2004 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
Cite this
A polysaccharide carrier for immunostimulatory CpG DNAs to enhance cytokine secretion. / Mizu, Masami; Koumoto, Kazuya; Anada, Takahisa; Matsumoto, Takahiro; Numata, Munenori; Shinkai, Seiji; Nagasaki, Takeshi; Sakurai, Kazuo.
In: Journal of the American Chemical Society, Vol. 126, No. 27, 14.07.2004, p. 8372-8373.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A polysaccharide carrier for immunostimulatory CpG DNAs to enhance cytokine secretion
AU - Mizu, Masami
AU - Koumoto, Kazuya
AU - Anada, Takahisa
AU - Matsumoto, Takahiro
AU - Numata, Munenori
AU - Shinkai, Seiji
AU - Nagasaki, Takeshi
AU - Sakurai, Kazuo
PY - 2004/7/14
Y1 - 2004/7/14
N2 - A β-(1 → 3)-D-glucan schizophyllan (SPG) forms a stoichiometric complex with some polynucleotides. This communication describes our attempt to apply the SPG complex to deliver CpG DNA to endosomes to enhance cytokine secretion. To increase cellular uptake, we introduced spermine, arginine-glycine-aspartic acid tripeptide, octaarginine, or cholesterol to the SPG side chain. The chemically modified SPG showed essentially no cytotoxicity. When CpG DNA complex made therefrom was exposed to macrophages, dramatic enhancement in the cytokine secretion was observed. It increased 5-10 times from the naked dose and 100 times from the background. This performance promises that SPG can be an excellent carrier for CpG DNA.
AB - A β-(1 → 3)-D-glucan schizophyllan (SPG) forms a stoichiometric complex with some polynucleotides. This communication describes our attempt to apply the SPG complex to deliver CpG DNA to endosomes to enhance cytokine secretion. To increase cellular uptake, we introduced spermine, arginine-glycine-aspartic acid tripeptide, octaarginine, or cholesterol to the SPG side chain. The chemically modified SPG showed essentially no cytotoxicity. When CpG DNA complex made therefrom was exposed to macrophages, dramatic enhancement in the cytokine secretion was observed. It increased 5-10 times from the naked dose and 100 times from the background. This performance promises that SPG can be an excellent carrier for CpG DNA.
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UR - http://www.scopus.com/inward/citedby.url?scp=3142676634&partnerID=8YFLogxK
U2 - 10.1021/ja031978+
DO - 10.1021/ja031978+
M3 - Article
C2 - 15237982
AN - SCOPUS:3142676634
VL - 126
SP - 8372
EP - 8373
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
SN - 0002-7863
IS - 27
ER -